The generation of adipocytes by the neural crest.
The generation of adipocytes by the neural crest.
复制标题
DOI：
10.1242/dev.002642
复制
发表时间：
2007-06
期刊：
影响因子：
--
通讯作者：
Dupin E
中科院分区：
文献类型：
--
作者：
Billon N;Iannarelli P;Monteiro MC;Glavieux-Pardanaud C;Richardson WD;Kessaris N;Dani C;Dupin E
脂肪细胞(脂肪细胞)由脂肪细胞前体细胞(前体脂肪细胞)发育而来，而脂肪细胞前体细胞本身来自间充质祖细胞。虽然控制前脂肪细胞分化为成熟脂肪细胞的事件已经被很大程度地探索了，但引导间充质祖细胞沿着脂肪细胞途径的机制仍然不清楚。同样，尽管脂肪细胞通常被认为来自中胚层，但关于胚胎发育过程中脂肪组织的起源和发育缺乏关键信息。这项研究的目的是深入了解脂肪细胞的个体发育，无论是在小鼠胚胎干细胞来源的培养中还是在正常发育过程中。我们首次使用基因工程的MES细胞来产生和选择ES细胞来源的神经上皮祖细胞，并表明在MES细胞来源的培养中，神经外胚层而不是中胚层可能是脂肪细胞的来源。然后，我们使用发育中的鹌鹑神经脊(NC)细胞的原代和继代培养来证明NC细胞在特定因素的刺激下能够分化为脂肪细胞，从而为研究脂肪细胞分化的早期阶段提供了一个强大的系统。最后，我们利用Cre介导的转基因小鼠体内重组定位了NC衍生物，并证明了在正常发育过程中，有一部分脂肪细胞起源于NC。
Fat cells (adipocytes) develop from adipocyte precursor cells (preadipocytes) that themselves derive from mesenchymal progenitors. Although the events controlling preadipocyte differentiation into mature adipocytes have been largely explored, the mechanisms that direct mesenchymal progenitors down the adipocyte pathway remain unknown. Similarly, although adipocytes are generally thought to derive from mesoderm, key information is lacking regarding the origin and the development of the adipose tissue during embryogenesis. The aim of this study was to gain insight into the ontogeny of fat cells, both in mouse embryonic stem (mES) cell-derived cultures and during normal development. We first used genetically engineered mES cells to produce and select ES cell-derived neuroepithelial progenitors and showed that neuroectoderm, rather than mesoderm, may be a source of adipocytes in mES cell-derived cultures. We then used primary and secondary cultures of developing quail neural crest (NC) cells to demonstrate that NC cells are able, upon stimulation with defined factors, to differentiate into adipocytes, thus providing a powerful system to study the earliest stages of adipocyte differentiation. Finally, we mapped NC derivatives in vivo using Cre-mediated recombination in transgenic mice and demonstrated that a subset of adipocytes originates from the NC during normal development.
登录
查看更多内容
影响因子：
6.5
作者：
Abderrahim-Ferkoune, A;Bezy, O;Amri, EZ
通讯作者：
Amri, EZ
影响因子：
16.2
作者：
DULAC, C;CAMERONCURRY, P;LEDOUARIN, NM
通讯作者：
LEDOUARIN, NM
影响因子：
2.7
作者：
BAIN, G;KITCHENS, D;GOTTLIEB, DI
通讯作者：
GOTTLIEB, DI
DOI：
10.1073/pnas.85.14.5325
发表时间：
1988-07-01
影响因子：
11.1
作者：
BAROFFIO, A;DUPIN, E;LEDOUARIN, NM
通讯作者：
LEDOUARIN, NM
影响因子：
11.4
作者：
Billon, N;Jolicoeur, C;Raff, M
通讯作者：
Raff, M