Involvement of flagella-driven motility and pili in Pseudomonas aeruginosa colonization at the air-liquid interface.

Involvement of flagella-driven motility and pili in Pseudomonas aeruginosa colonization at the air-liquid interface.
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DOI:
10.1264/jsme2.me11322
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发表时间:
2012
影响因子:
2.2
通讯作者:
Igarashi Y
Igarashi Y
中科院分区:
环境科学与生态学4区
文献类型:
--
作者:
Yamamoto K;Arai H;Ishii M;Igarashi Y

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许多好氧微生物可以在气液界面上定居,并形成一种多细胞结构,称为膜层。本研究探讨了运动特性和附着特性在铜绿假单胞菌膜形成过程中的作用。鞭毛和鞭毛运动缺陷突变体表现出膜形成延迟和膜形态异常,表明鞭毛驱动的运动性对膜结构的发育有很大贡献。一个菌毛缺陷突变体表现出正常的膜形成特性,而鞭毛缺陷突变体中的菌毛基因的破坏恢复了正常的膜形态。这些结果表明鞭毛和菌毛在铜绿假单胞菌膜的发育过程中起着关键作用。
Many aerobic microorganisms can colonize at the air-liquid interface and form a multicellular structure, known as a pellicle. In this study, the involvement of motility and attachment traits in the Pseudomonas aeruginosa pellicle formation process was investigated. Flagella- and flagellar-motor-deficient mutants exhibited delayed pellicle formation and unusual pellicle morphology, indicating the large contribution of flagella-driven motility to structural development of the pellicle. A pili-deficient mutant showed normal pellicle formation properties, while the disruption of the pilus gene in the flagella-deficient mutant restored normal pellicle morphology. These results indicate that flagella and pili play key roles in P. aeruginosa pellicle development.
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发表时间: 2008-09-01
影响因子: 5.1
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