Open source tracking and analysis of adult Drosophila locomotion in Buridan's paradigm with and without visual targets.
Open source tracking and analysis of adult Drosophila locomotion in Buridan's paradigm with and without visual targets.
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DOI：
10.1371/journal.pone.0042247
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Brembs B
中科院分区：
文献类型：
--
作者：
Colomb J;Reiter L;Blaszkiewicz J;Wessnitzer J;Brembs B
昆虫一直是研究神经系统控制运动的最广泛使用的模型系统之一。在果蝇中，我们对运动进行了一个简单的测试：在布里丹的范例中，苍蝇在两个无法接近的视觉目标之间来回行走。直到今天，缺乏容易获得的工具来跟踪苍蝇的位置和分析其轨迹可能有助于缓慢接受布里丹的范式。我们在这里提出了一个开源软件包，旨在跟踪一个单一的动物行走在一个同质的环境（Buritrack），并分析其轨迹。质心轨迹分析（CeTrAn）软件在开源统计项目R中编码。它提取了11个指标，包括相关性分析和主成分分析（PCA）。它被设计成可以很容易地根据个人需求进行定制。结合廉价的硬件，这些工具可以很容易地用于教学和研究目的。我们证明了我们的包的能力，通过测量成年果蝇（其翅膀被剪）的运动行为，无论是在存在或不存在的视觉目标，并比较后者不同的计算机生成的数据。轨迹的分析证实，苍蝇是centrophobic，并表明，无法访问的视觉目标可以改变苍蝇的方向，而不改变其整体模式的活动。使用计算机生成的数据，对分析软件进行测试，并设置一些度量的机会值（以及它们的相关性的机会值）。我们的研究结果提示的假设，只有当负趋光性可以克服苍蝇的倾向，以避免平台的中心，观察到的固定行为。与我们的配套文件一起，我们提供了新的工具来促进开放科学以及数字行为数据的收集和分析。
Insects have been among the most widely used model systems for studying the control of locomotion by nervous systems. In Drosophila, we implemented a simple test for locomotion: in Buridan's paradigm, flies walk back and forth between two inaccessible visual targets. Until today, the lack of easily accessible tools for tracking the fly position and analyzing its trajectory has probably contributed to the slow acceptance of Buridan's paradigm. We present here a package of open source software designed to track a single animal walking in a homogenous environment (Buritrack) and to analyze its trajectory. The Centroid Trajectory Analysis (CeTrAn) software is coded in the open source statistics project R. It extracts eleven metrics and includes correlation analyses and a Principal Components Analysis (PCA). It was designed to be easily customized to personal requirements. In combination with inexpensive hardware, these tools can readily be used for teaching and research purposes. We demonstrate the capabilities of our package by measuring the locomotor behavior of adult Drosophila melanogaster (whose wings were clipped), either in the presence or in the absence of visual targets, and comparing the latter to different computer-generated data. The analysis of the trajectories confirms that flies are centrophobic and shows that inaccessible visual targets can alter the orientation of the flies without changing their overall patterns of activity. Using computer generated data, the analysis software was tested, and chance values for some metrics (as well as chance value for their correlation) were set. Our results prompt the hypothesis that fixation behavior is observed only if negative phototaxis can overcome the propensity of the flies to avoid the center of the platform. Together with our companion paper, we provide new tools to promote Open Science as well as the collection and analysis of digital behavioral data.
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影响因子：
4.3
作者：
Sorribes A;Armendariz BG;Lopez-Pigozzi D;Murga C;de Polavieja GG
通讯作者：
de Polavieja GG
DOI：
10.1007/s003590050190
发表时间：
1998-04-01
期刊：
JOURNAL OF COMPARATIVE PHYSIOLOGY A-SENSORY NEURAL AND BEHAVIORAL PHYSIOLOGY
影响因子：
--
作者：
Strauss, R;Pichler, J
通讯作者：
Pichler, J
影响因子：
1.3
作者：
Martin, JR
通讯作者：
Martin, JR
影响因子：
1.9
作者：
Serway, Christine N.;Kaufman, Rebecca R.;de Belle, J. Steven
通讯作者：
de Belle, J. Steven
影响因子：
--
作者：
McEwen, RS
通讯作者：
McEwen, RS