Enhancing resin-dentin bond durability using a novel mussel-inspired monomer.
Enhancing resin-dentin bond durability using a novel mussel-inspired monomer.
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使用新型贻贝单体增强树脂-牙本质粘合耐久性
DOI：
10.1016/j.mtbio.2021.100174
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发表时间：
2021-09
期刊：
影响因子：
--
通讯作者：
Yiu CKY
中科院分区：
文献类型：
--
作者：
Li K;Yao C;Sun Y;Wang K;Wang X;Wang Z;Tsoi JKH;Huang C;Yiu CKY
人们已经开发了许多方法来改善树脂-牙本质粘合性能，其中贻贝源化合物的生物应用最近引起了人们的广泛关注。为了评估 N-(3,4-二羟基苯乙基)甲基丙烯酰胺 (DMA)（一种贻贝衍生化合物）作为牙科粘合剂中的功能单体的性能，首先通过透射电子显微镜 (TEM)、傅里叶变换红外衰减全反射技术 (ATR-FTIR) 评估其与牙本质胶原蛋白交联并与粘合剂聚合的潜在特性， 以及通过 Peakforce QNM 模式的原子力显微镜 (AFM)。在分别验证DMA对胶原蛋白和粘合剂的影响后，通过微拉伸粘合强度（μTBS）测试、断裂模式观察和纳米渗漏评估（即刻和10,000次热循环老化后）来检查DMA/乙醇溶液作为牙本质粘合底漆的整体性能。使用共焦激光扫描显微镜（CLSM）通过原位酶谱法评估DMA对内源金属蛋白酶（MMP）的抑制作用，并使用细胞计数试剂盒8评估DMA的细胞毒性。结果表明，DMA 通过非共价键成功与牙本质胶原交联，并且对粘合剂的聚合和机械性能没有影响。此外，即使经过 10,000 次热循环老化，DMA 处理组的 μTBS 和 nanoleakage 表达与其即时值相比也没有显示出显着变化。原位酶谱显示应用 DMA 后内源蛋白水解活性降低，并且 DMA 浓度高达 25 μmol/L 时未观察到细胞毒性作用。因此，DMA可以用作牙本质粘合中的新型生物相容性功能单体。 DMA作为牙本质粘接系统的功能单体，具有高生物相容性。 DMA连接粘合剂和胶原网络以抵抗各种外部攻击。 DMA/乙醇抑制 MMP 的活性并提高树脂-牙本质粘合的耐久性。
Numerous approaches have been developed to improve the resin-dentin bond performance, among which the bio-application of mussel-derived compounds have drawn great attention recently. To assess the performance of N-(3,4-dihydroxyphenethyl)methacrylamide (DMA), a mussel-derived compound, as a functional monomer in dental adhesive, its potential property to cross-link with dentin collagen and polymerize with adhesive will first be evaluated by transmission electron microscopy (TEM), attenuated total reflectance technique of Fourier transform infrared (ATR-FTIR), and atomic force microscopy (AFM) via Peakforce QNM mode. After validating the influence of DMA on collagen and adhesive separately, the overall performance of DMA/ethanol solution as a primer in dentin bonding was examined using micro-tensile bond strength (μTBS) testing, fracture pattern observation, and nanoleakage evaluation both immediately and after 10,000 times thermocycling aging. The inhibitory effect of DMA on endogenous metalloproteinases (MMPs) was evaluated by in situ zymography using confocal laser scanning microscopy (CLSM) and the cytotoxicity of DMA was evaluated using cell counting kit-8. Results demonstrated that DMA successfully cross-linked with dentin collagen via non-covalent bonds and had no influence on the polymerization and mechanical properties of the adhesive. Furthermore, even after 10,000 times thermocycling aging, the μTBS and nanoleakage expression of the DMA-treated groups showed no significant change compared with their immediate values. In situ zymography revealed reduced endogenous proteolytic activities after the application of DMA, and no cytotoxicity effect was observed for DMA concentration up to 25 μmol/L. Thus, DMA could be used as a novel, biocompatible functional monomer in dentin bonding. DMA acts as a functional monomer in dentin bonding system with high biocompatibility. DMA connects the adhesive and collagen network to resist various external attacks. DMA/ethanol inhibits the activity of MMPs and improve resin-dentin bond durability.
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DOI：
10.1002/jbm.b.31095
发表时间：
2009-02
影响因子：
3.4
作者：
Kostoryz, Elisabet L.;Dharmala, Kiran;Ye, Qiang;Wang, Yong;Huber, Jesse;Park, Jong-Gu;Snider, Grant;Katz, J. Lawrence;Spencer, Paulette
通讯作者：
Spencer, Paulette
影响因子：
14
作者：
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通讯作者：
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影响因子：
4.4
作者：
Deng, Donglai;Yang, Hongye;Huang, Cui
通讯作者：
Huang, Cui
影响因子：
9.7
作者：
Lee BP;Messersmith PB;Israelachvili JN;Waite JH
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影响因子：
6.2
作者：
Lee SB;González-Cabezas C;Kim KM;Kim KN;Kuroda K
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