Enzymatic biodegradation of HEMA/bisGMA adhesives formulated with different water content.
Enzymatic biodegradation of HEMA/bisGMA adhesives formulated with different water content.
复制标题
DOI：
10.1002/jbm.b.31095
复制
发表时间：
2009-02
影响因子：
3.4
通讯作者：
Spencer, Paulette
中科院分区：
文献类型：
--
作者：
Kostoryz, Elisabet L.;Dharmala, Kiran;Ye, Qiang;Wang, Yong;Huber, Jesse;Park, Jong-Gu;Snider, Grant;Katz, J. Lawrence;Spencer, Paulette
牙本质粘合剂在粘合到湿的脱矿质牙本质时可能会发生相分离。我们假设表现出相分离的粘合剂将经历增强的甲基丙烯酸酯基团的生物降解。该项目的目的是研究酶暴露对模拟湿粘合条件下配制的粘合剂中甲基丙烯酸 (MAA) 和甲基丙烯酸 2-羟乙酯 (HEMA) 释放的影响。 HEMA/bisGMA(2,2-双[4(2-羟基-3-甲基丙烯酰氧基-丙氧基)-苯基]丙烷)，45/55 w/w 比例，以不同的水含量配制：0 wt% (A00)、8 wt% (A08) 和 16 wt% (A16)。预清洗三天后，将粘合盘在磷酸盐缓冲液（PB，pH 7.4）中在有/无猪肝酯酶（PLE）的情况下在 37°C 下孵育 8 天。每天收集上清液并通过 HPLC 分析 MAA 和 HEMA。对于所有制剂，与 PB 中的 MAA 释放相比，PLE 存在下的每日 MAA 释放增加。在 PLE 存在的情况下未检测到 HEMA 释放，而在 PB 中始终测量到 HEMA 释放。与 A00 相比，A08 和 A16 释放的 HEMA 量显着增加。对分析物累积释放的分析表明，与 PB 中释放的可浸出物相比，PLE 中的可浸出物显着增加 (p < 0.05)，这表明 MAA 释放不仅由未反应的单体形成，而且由聚合物网络中的侧基形成。然而，与 A00 相比，A08 和 A16 中 PB 中的分析物 HEMA 或 PLE 中的 MAA 水平有所增加，这表明在湿粘合条件下经历相分离的 HEMA/bisGMA 粘合剂中的材料损失可能更大。
Dentin adhesives may undergo phase separation when bonding to wet demineralized dentin. We hypothesized that adhesives exhibiting phase separation will experience enhanced biodegradation of methacrylate ester groups. The objective of this project was to study the effect of enzyme-exposure on the release of methacrylic acid (MAA) and 2-hydroxyethyl methacrylate (HEMA) from adhesives formulated under conditions simulating wet bonding. HEMA/bisGMA(2,2-bis[4(2-hydroxy-3-methacryloyloxy-propyloxy)-phenyl] propane), 45/55 w/w ratio, was formulated with different water content: 0 Wt % (A00), 8 wt % (A08), and 16 wt % (A16). After a three day prewash, adhesive discs were incubated with/without porcine liver esterase (PLE) in phosphate buffer (PB, pH 7.4) at 37°C for 8 days. Supernatants were collected daily and analyzed for MAA and HEMA by HPLC. For all formulations, daily MAA release in the presence of PLE was increased compared to MAA release in PB. HEMA release in the presence of PLE was not detected while HEMA release was consistently measured in PB. A08 and A16 released significantly larger amounts of HEMA compared to A00. Analysis of the cumulative release of analytes showed that the leachables in PLE was significantly increased (p < 0.05) as compared with that released in PB indicating that MAA release was not only formed from unreacted monomers but from pendant groups in the polymer network. However, the levels of analytes HEMA in PB or MAA in PLE were increased in A08 and A16 as compared with A00, which suggests that there could be a greater loss of material in HEMA/bisGMA adhesives that experience phase separation under wet bonding conditions.
登录
查看更多内容
影响因子：
4.9
作者：
Finer, Y;Santerre, JP
通讯作者：
Santerre, JP
影响因子：
4.9
作者：
Finer, Y.;Santerre, J. P.
通讯作者：
Santerre, J. P.
影响因子：
7.6
作者：
Hashimoto, M;Ohno, H;Oguchi, H
通讯作者：
Oguchi, H
影响因子：
7.6
作者：
Eick, JD;Miller, RG;Chappelow, CC
通讯作者：
Chappelow, CC
影响因子：
14
作者：
Finer, Y;Jaffer, F;Santerre, JP
通讯作者：
Santerre, JP