Study on the chemotherapeutic effect and mechanism of cucurbitacin E on laryngeal cancer stem cells.
Study on the chemotherapeutic effect and mechanism of cucurbitacin E on laryngeal cancer stem cells.
复制标题
葫芦素E对喉癌干细胞的化疗作用及机制研究
DOI：
10.1002/lio2.773
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发表时间：
2022-06
影响因子：
1.9
通讯作者：
Zhang, Jingyue
中科院分区：
文献类型：
--
作者：
Jiang, Xuelian;Ma, Binjuan;Wei, Xudong;Xu, Xiaoyan;Cao, Jiyan;Zhang, Jingyue
葫芦素E对喉癌干细胞的化疗作用及其机制研究采用流式细胞仪分选CD133+喉癌干细胞，台盼蓝拒染法检测喉癌干细胞的存活率，细胞计数试剂盒8(CCK-8)检测CUE对干细胞增殖能力的影响及阿霉素的化疗增强作用，Transwell实验观察CUE对干细胞迁移能力的影响，Western Blot检测CUE对干细胞相关蛋白表达水平的影响。实验结束后测量裸鼠肿瘤体积，制备裸鼠肿瘤组织石蜡切片，苏木精伊红(H&E)染色。免疫组织化学方法检测裸鼠肿瘤组织中c-myc的表达，Western Blot法检测CuE对裸鼠肿瘤组织中相关蛋白表达水平的影响。CUE在体外可降低喉癌干细胞的存活率、增殖能力和迁移能力，且对阿霉素有化疗增强作用。CUE的化疗作用机制可能是降低c-myc蛋白的表达，而化疗协同作用的机制可能是降低ABCG2和P-gp蛋白的表达。CUE对喉癌干细胞有化疗作用，也有化疗增效作用。葫芦素E对喉癌干细胞的化疗作用及其机制研究
Study on the chemotherapeutic effect and mechanism of cucurbitacin E (CuE) on laryngeal cancer stem cells. We used flow cytometry to sort out CD133+ laryngeal cancer stem cells; trypan blue rejection assay to detect the survival rate of laryngeal cancer stem cells; Cell counting kit‐8 (CCK‐8) assay to detect the effect of CuE on the proliferation ability of stem cells and the chemotherapeutic potentiation of doxorubicin; Transwell assay to observe the effect of CuE on the migration ability of stem cells; and Western Blot to detect the effect of CuE on the expression level of stem cell‐associated proteins. The tumor volume of nude mice was measured at the end of the experiment, and paraffin sections of nude mice tumor tissues were prepared and stained with Hematoxylin and eosin (H&E). The expression of c‐MYC in tumor tissues of nude mice was further detected by immunohistochemistry, and the effect of CuE on the expression level of related proteins in tumor tissues of nude mice was detected by Western Blot. CuE reduced the survival rate, proliferation ability, and migration ability of laryngeal cancer stem cells in vitro, and that CuE had a chemotherapeutic potentiating effect on doxorubicin. The possible mechanism of the chemotherapeutic effect of CuE was to reduce the expression of c‐MYC protein, and the possible mechanism of chemotherapy synergy was to reduce the expression of ABCG2 and P‐gp protein. CuE has a chemotherapeutic effect on laryngeal cancer stem cells, as well as a chemotherapy synergy. Study on the chemotherapeutic effect and mechanism of cucurbitacin E on laryngeal cancer stem cells.
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影响因子：
--
作者：
Wei, Xudong;He, Jian;Wang, Wei
通讯作者：
Wang, Wei
影响因子：
5
作者：
Szafarowski, Tomasz;Sierdzinski, Janusz;Szczepanski, Miroslaw J.
通讯作者：
Szczepanski, Miroslaw J.
影响因子：
4
作者：
Wang Y;Xu S;Wu Y;Zhang J
通讯作者：
Zhang J
影响因子：
7
作者：
Cheng YM;Shen CJ;Chang CC;Chou CY;Tsai CC;Hsu YC
通讯作者：
Hsu YC
DOI：
10.12182/20200960503
发表时间：
2020-09-01
期刊：
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
影响因子：
--
作者：
Chen, Chen;Xu, Zi-Han;Wang, Li
通讯作者：
Wang, Li