De Novo Analysis of the Transcriptome of Meloidogyne enterolobii to Uncover Potential Target Genes for Biological Control.
De Novo Analysis of the Transcriptome of Meloidogyne enterolobii to Uncover Potential Target Genes for Biological Control.
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对肠根结线虫转录组进行从头分析，以发现生物防治的潜在靶基因
DOI：
10.3390/ijms17091442
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发表时间：
2016-09-01
影响因子：
5.6
通讯作者：
Jian H
中科院分区：
文献类型：
--
作者：
Li X;Yang D;Niu J;Zhao J;Jian H
肠根结线虫是专性生物营养性根结线虫之一，具有在许多重要经济作物上繁殖的能力。我们使用Roche GS FLX对enterolobii的转录组进行了从头测序，获得了408,663个高质量的reads，组装成8193个contigs和31,860个singleton。我们比较了作为生物防治潜在目标的不同线虫的转录本。这些转录本包括被认为编码CAZymes、激酶、神经肽基因和分泌蛋白的转录本，以及参与RNAi途径和免疫信号传导的转录本。通常，75种非膜分泌蛋白与食管癌细胞分泌的信号肽被确定为可能的效应蛋白，其中3种蛋白通过基于PVX （pGR107）的benthamiana （N. benthamiana）的高通量瞬时植物表达系统进行了初步检测。结果表明，这些候选蛋白可抑制促凋亡蛋白BAX触发的程序性细胞死亡（PCD），其中一种蛋白还可引起细胞坏死，提示它们可能抑制植物免疫应答，促进致病性。综上所述，本研究首次全面了解了肠芽孢杆菌的转录组，为进一步研究和制定生物防治策略奠定了基础。
Meloidogyne enterolobii is one of the obligate biotrophic root-knot nematodes that has the ability to reproduce on many economically-important crops. We carried out de novo sequencing of the transcriptome of M. enterolobii using Roche GS FLX and obtained 408,663 good quality reads that were assembled into 8193 contigs and 31,860 singletons. We compared the transcripts in different nematodes that were potential targets for biological control. These included the transcripts that putatively coded for CAZymes, kinases, neuropeptide genes and secretory proteins and those that were involved in the RNAi pathway and immune signaling. Typically, 75 non-membrane secretory proteins with signal peptides secreted from esophageal gland cells were identified as putative effectors, three of which were preliminarily examined using a PVX (pGR107)-based high-throughput transient plant expression system in Nicotiana benthamiana (N. benthamiana). Results showed that these candidate proteins suppressed the programmed cell death (PCD) triggered by the pro-apoptosis protein BAX, and one protein also caused necrosis, suggesting that they might suppress plant immune responses to promote pathogenicity. In conclusion, the current study provides comprehensive insight into the transcriptome of M. enterolobii for the first time and lays a foundation for further investigation and biological control strategies.
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DOI：
10.1093/bioinformatics/btt419
发表时间：
2013-10-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Goldberg JM;Griggs AD;Smith JL;Haas BJ;Wortman JR;Zeng Q
通讯作者：
Zeng Q
影响因子：
3.5
作者：
Jaouannet, M.;Magliano, M.;Rosso, M. N.
通讯作者：
Rosso, M. N.
影响因子：
7.2
作者：
Jamir, Y;Guo, M;Alfano, JR
通讯作者：
Alfano, JR
DOI：
10.1093/bioinformatics/btp348
发表时间：
2009-08-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Capella-Gutiérrez S;Silla-Martínez JM;Gabaldón T
通讯作者：
Gabaldón T
影响因子：
11.4
作者：
Abramovitch, RB;Kim, YJ;Martin, GB
通讯作者：
Martin, GB