Accelerating bacterial growth detection and antimicrobial susceptibility assessment in integrated picoliter droplet platform.
Accelerating bacterial growth detection and antimicrobial susceptibility assessment in integrated picoliter droplet platform.
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DOI：
10.1016/j.bios.2017.06.006
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发表时间：
2017-11-15
影响因子：
12.6
通讯作者：
Wang TH
中科院分区：
文献类型：
--
作者：
Kaushik AM;Hsieh K;Chen L;Shin DJ;Liao JC;Wang TH
仍然迫切需要快速诊断方法，可以评估病原菌的抗生素耐药性，以提供有针对性的抗生素治疗。为此，我们提出了一个快速和集成的单细胞生物传感平台，称为dropFAST，用于细菌生长检测和抗菌药物敏感性评估。DropFAST利用基于刃天青的快速荧光生长测定，结合细菌在20 μ L液滴中的随机限制，以在孵育1小时后检测来自生长细菌的信号，相当于2-3次细菌复制。将液滴生成、孵育和检测完全集成到一个单一的不间断流中也使该平台特别适合在线细菌表型生长评估。为了说明快速数字化抗菌药物敏感性评估的概念，我们使用dropFAST平台来评估庆大霉素对E.大肠杆菌生长。
There remains an urgent need for rapid diagnostic methods that can evaluate antibiotic resistance for pathogenic bacteria in order to deliver targeted antibiotic treatments. Toward this end, we present a rapid and integrated single-cell biosensing platform, termed dropFAST, for bacterial growth detection and antimicrobial susceptibility assessment. DropFAST utilizes a rapid resazurin-based fluorescent growth assay coupled with stochastic confinement of bacteria in 20 pL droplets to detect signal from growing bacteria after 1 h incubation, equivalent to 2–3 bacterial replications. Full integration of droplet generation, incubation, and detection into a single, uninterrupted stream also renders this platform uniquely suitable for in-line bacterial phenotypic growth assessment. To illustrate the concept of rapid digital antimicrobial susceptibility assessment, we employ the dropFAST platform to evaluate the antibacterial effect of gentamicin on E. coli growth.
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影响因子：
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