Selection and Validation of Novel RT-qPCR Reference Genes under Hormonal Stimuli and in Different Tissues of Santalum album.
Selection and Validation of Novel RT-qPCR Reference Genes under Hormonal Stimuli and in Different Tissues of Santalum album.
复制标题
激素刺激下和檀香不同组织中新型 RT-qPCR 参考基因的选择和验证
DOI：
10.1038/s41598-018-35883-6
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发表时间：
2018-11-30
影响因子：
4.6
通讯作者：
Ma G
中科院分区：
文献类型：
--
作者：
Yan H;Zhang Y;Xiong Y;Chen Q;Liang H;Niu M;Guo B;Li M;Zhang X;Li Y;Teixeira da Silva JA;Ma G
Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) is a widely used technique to investigate gene expression levels due to its high throughput, specificity, and sensitivity. An appropriate reference gene is essential for RT-qPCR analysis to obtain accurate and reliable results. To date, no reliable reference gene has been validated for the economically tropical tree, sandalwood (Santalum albumL.). In this study, 13 candidate reference genes, including 12 novel putative reference genes selected from a large set ofS.albumtranscriptome data, as well as the currently used β-actin gene (ACT), were validated in different tissues (stem, leaf, root and callus), as well as callus tissue under salicylic acid (SA), jasmonic acid methyl ester (MeJA), and gibberellin (GA) treatments using geNorm, NormFinder, BestKeeper, Delta Ct and comprehensive RefFinder algorithms. Several novel candidate reference genes were much more stable than the currently used traditional geneACT.ODDpaired withFbp1for SA treatment,CSAandFbp3for MeJA treatment,PP2CandFbp2for GA treatment, as well asFbp1combined withFbp2for the total of three hormone treatments were the most accurate reference genes, respectively.FAB1A, when combined withPP2C, was identified as the most suitable reference gene combination for the four tissues tested, while the combination ofHLMt,PPRandFAB1Awere the most optimal reference genes for all of the experimental samples. In addition, to verify our results, the relative expression level of theSaSSygene was evaluated by the validated reference genes and their combinations in the threeS.albumtissues and under MeJA treatment. The evaluated reference genes in this study will improve the accuracy of RT-qPCR analysis and will benefitS.albumfunctional genomics studies in different tissues and under hormone stimuli in the future.
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影响因子：
1.5
作者：
Maroufi, A.
通讯作者：
Maroufi, A.
影响因子：
3.7
作者：
de Jonge HJ;Fehrmann RS;de Bont ES;Hofstra RM;Gerbens F;Kamps WA;de Vries EG;van der Zee AG;te Meerman GJ;ter Elst A
通讯作者：
ter Elst A
选择并验证雄花同株太行花雄花和完美花实时定量 PCR 标准化的内参基因
影响因子：
5.6
作者：
Li W;Zhang L;Zhang Y;Wang G;Song D;Zhang Y
通讯作者：
Zhang Y
影响因子：
3.7
作者：
Liu J;Wang Q;Sun M;Zhu L;Yang M;Zhao Y
通讯作者：
Zhao Y
影响因子：
6.4
作者：
MURASHIGE, T;SKOOG, F
通讯作者：
SKOOG, F