Genes as early responders regulate quorum-sensing and control bacterial cooperation in Pseudomonas aeruginosa.
Genes as early responders regulate quorum-sensing and control bacterial cooperation in Pseudomonas aeruginosa.
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DOI：
10.1371/journal.pone.0101887
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Wu M
中科院分区：
文献类型：
--
作者：
Zhao K;Li Y;Yue B;Wu M
群体感应（Quorum-sensing，QS）允许细菌通信以协调细胞外产物的产生，所述细胞外产物对于在较高细胞密度下的群体适应性是必需的。人们普遍认为，需要相当长的时间才能达到适当的细胞密度，以激活编码这些昂贵但有益的公共产品的相关静止基因。涉及哪些调控基因以及这些基因如何在早期阶段控制细菌通讯在很大程度上尚未探索。通过测定条件致病菌铜绿假单胞菌QS相关基因的时间依赖性表达，我们表明，社会合作的诱导可能受到环境因素的严重影响，以优化人口密度。特别是，作为早期应答者的小调控RNA（RsmY和RsmZ）可以促进依赖基因（例如lasR）的表达，以促进细胞内酶的合成并协调细菌细胞中的即时合作行为。这些早期反应者，作为一个变阻器，精细地调节细菌的合作，这可能会迅速激活环境威胁下，但彼得关闭时，关键的QS依赖基因是完全功能的合作。我们的研究结果表明，RsmY和RsmZ严格控制的时间和水平的公共产品生产，这可能会在社会微生物学和感染控制的影响。
Quorum-sensing (QS) allows bacterial communication to coordinate the production of extracellular products essential for population fitness at higher cell densities. It has been generally accepted that a significant time duration is required to reach appropriate cell density to activate the relevant quiescent genes encoding these costly but beneficial public goods. Which regulatory genes are involved and how these genes control bacterial communication at the early phases are largely un-explored. By determining time-dependent expression of QS-related genes of the opportunistic pathogen Pseudomonas aerugionsa, we show that the induction of social cooperation could be critically influenced by environmental factors to optimize the density of population. In particular, small regulatory RNAs (RsmY and RsmZ) serving as early responders, can promote the expression of dependent genes (e.g. lasR) to boost the synthesis of intracellular enzymes and coordinate instant cooperative behavior in bacterial cells. These early responders, acting as a rheostat to finely modulate bacterial cooperation, which may be quickly activated under environment threats, but peter off when critical QS dependent genes are fully functional for cooperation. Our findings suggest that RsmY and RsmZ critically control the timing and levels of public goods production, which may have implications in sociomicrobiology and infection control.
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