Non-esterified fatty acid-associated ability of follicular fluid to support porcine oocyte maturation and development.
Non-esterified fatty acid-associated ability of follicular fluid to support porcine oocyte maturation and development.
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DOI：
10.1002/rmb2.12084
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发表时间：
2018-04
影响因子：
3.4
通讯作者：
Iwata H
中科院分区：
文献类型：
--
作者：
Ogawa K;Itami N;Ueda M;Kansaku K;Shirasuna K;Kuwayama T;Iwata H
根据卵泡液（FF）的非酯化脂肪酸（NEFA）含量或脂肪酸混合物（FA - Mix）对卵母细胞发育能力以及卵母细胞和卵囊细胞线粒体质量和数量的影响进行了研究。使用了屠宰场的猪卵母细胞。成熟培养基中的FF或FA‐Mix均能增加卵母细胞和卵丘细胞的脂质含量，但对三磷酸腺苷含量的影响不同。FF添加物增加了线粒体DNA拷贝数、卵囊细胞存活率和卵母细胞发育到囊胚期的速度，而FA‐Mix添加物没有显示出这些影响。与不添加FF的细胞相比，添加FF后积云细胞中GPC4、PFKP、PRDX3和TFAM的表达水平升高，而GJA1的表达水平降低。在成熟培养基中加入FF和FA‐Mix可提高卵母细胞和卵丘细胞的脂质含量。在补充FA‐Mix后，未观察到FF对卵丘细胞和卵母细胞的影响，这表明FF中NEFAs的浓度与支持卵丘细胞成熟和卵丘细胞和卵母细胞代谢的能力密切相关。
The effect of supplementing maturation medium with follicular fluid (FF) was examined according to its non‐esterified fatty acid (NEFA) content or with a fatty acid mixture (FA‐Mix) on the developmental competence of oocytes, as well as the mitochondrial quality and quantity in the oocytes and cumulus cells. Porcine oocytes from a slaughterhouse were used. The FF or FA‐Mix in maturation medium increased the lipid content in both the oocytes and the cumulus cells, but the adenosine triphosphate content was differentially affected. The FF supplementation increased the mitochondrial DNA copy number, survival of cumulus cells, and rate of oocyte development to the blastocyst stage, whereas the FA‐Mix supplementation did not show these effects. The expression levels of GPC4,PFKP,PRDX3, and TFAM in the cumulus cells increased after FF supplementation, but the expression of GJA1 decreased, compared with the cells that were cultured without FF. Adding FF and FA‐Mix to the maturation medium increased the lipid content in the oocytes and cumulus cells. The effects of FF on the cumulus cells and oocytes were not observed after FA‐Mix supplementation, indicating that the concentration of the NEFAs in the FF are closely associated with an ability to support oocyte maturation and the metabolism of cumulus cells and oocytes.
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暴露于富含脂质的卵泡液与卵丘-卵母细胞复合物中的内质网应激和卵母细胞成熟受损有关
影响因子：
6.7
作者：
Yang, Xing;Wu, Linda L.;Robker, Rebecca L.
通讯作者：
Robker, Rebecca L.
DOI：
10.1186/1477-7827-12-13
发表时间：
2014-02-05
期刊：
Reproductive biology and endocrinology : RB&E
影响因子：
--
作者：
Valckx SD;Arias-Alvarez M;De Pauw I;Fievez V;Vlaeminck B;Fransen E;Bols PE;Leroy JL
通讯作者：
Leroy JL
影响因子：
1.8
作者：
Yoshioka, Koji;Suzuki, Chie;Onishi, Akira
通讯作者：
Onishi, Akira
影响因子：
3.6
作者：
Sutton-McDowall, Melanie L.;Wu, Linda L. Y.;Robker, Rebecca L.
通讯作者：
Robker, Rebecca L.
影响因子：
3.6
作者：
Paczkowski, Melissa;Silva, Elena;Krisher, Rebecca L.
通讯作者：
Krisher, Rebecca L.