Identification of differentially expressed genes related to coloration in red/green mutant pear (Pyrus communis L.)
Identification of differentially expressed genes related to coloration in red/green mutant pear (Pyrus communis L.)
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红/绿突变梨（Pyrus communis L.）着色相关差异表达基因的鉴定
DOI：
10.1007/s11295-012-0534-3
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发表时间：
2013-02
期刊：
影响因子：
--
通讯作者：
Jun Wu, Guang Zhao, Ya-Nan Yang等
中科院分区：
文献类型：
--
作者：
Jun Wu, Guang Zhao, Ya-Nan Yang等
果皮颜色是果实外观品质的重要参数，对吸引顾客起着重要作用。在许多植物中，它是花色素苷途径基因协调调控的结果。本研究以梨的一对红色和绿色突变体为材料，对差异表达的cDNA文库进行分析，寻找可能调控花色素苷合成和控制梨着色的候选基因。利用cDNA扩增片段长度多态性（cDNA amplified fragment length polymorphism）技术构建了一个cDNA文库，分析了果皮高花青素含量的早红Doyenne du Comice及其低花青素含量的绿色突变体的转录差异。共鉴定出47个转录本衍生片段，涉及花色素苷生物合成、初级代谢、胁迫和防御反应。采用实时荧光定量PCR技术，对不同发育时期的果皮样品进行了差异表达基因与果实着色的关系研究。发现了一个参与花青苷生物合成的基因PyMADS18。它的序列与文献中报道的花青素苷生物合成调节基因相似。表达结果表明，PyMADS18可能参与梨果实发育早期花青苷的积累和调控。
Fruit skin color is an important parameter of outer quality and plays an important role in attracting customers. In many plants, it is the result of coordinative regulation of anthocyanin pathway genes. In our study, the differential expression of cDNA library in a pair of pear mutant with red and green color was investigated to find candidate genes which might regulate the anthocyanin biosynthesis and control the coloration of pear. We constructed a cDNA library using the cDNA-amplified fragment length polymorphism approach to analyze the transcriptional differences between the original cultivar “Early red Doyenne du Comice” with high anthocyanin content in the peel and its green color mutant with comparatively low anthocyanin content. Altogether, 47 transcript-derived fragments, putatively involved in anthocyanin biosynthesis, primary metabolism, stress, and defense responses, were identified. The relationships of differentially expressed genes and coloration were investigated by quantitative real-time PCR with fruit skin samples at different developmental stages. A gene putatively involved in anthocyanin biosynthesis was found and named as PyMADS18. Its sequence is similar to genes reported in the literature as regulators of anthocyanin biosynthesis. The expression results indicate that PyMADS18 is likely to be involved in anthocyanin accumulation and regulation of anthocyanin synthesis in early fruit development of pear.
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DOI：
10.21273/jashs.129.1.0006
发表时间：
2004
影响因子：
1.9
作者：
W. Steyn;D. Holcroft;S. Wand;G. Jacobs
通讯作者：
W. Steyn;D. Holcroft;S. Wand;G. Jacobs
DOI：
10.1111/j.1365-313x.2006.02964.x
发表时间：
2007-02
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Espley RV;Hellens RP;Putterill J;Stevenson DE;Kutty-Amma S;Allan AC
通讯作者：
Allan AC
F-box蛋白家族。
影响因子：
12.3
作者：
Kipreos, E T;Pagano, M
通讯作者：
Pagano, M
DOI：
--
发表时间：
2009
期刊：
Food Science
影响因子：
--
作者：
Xin Guang
通讯作者：
Xin Guang
影响因子：
5.1
作者：
K. Okada;Y. Shimura
通讯作者：
K. Okada;Y. Shimura