Quantitative characterization of electron detectors for transmission electron microscopy.

Quantitative characterization of electron detectors for transmission electron microscopy.
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DOI:
10.1016/j.jsb.2013.10.016
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发表时间:
2013-12
影响因子:
3
通讯作者:
Grigorieff, Nikolaus
Grigorieff, Nikolaus
中科院分区:
生物学3区
文献类型:
--
作者:
Ruskin, Adrian I.;Yu, Zhiheng;Grigorieff, Nikolaus

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新一代直接电子探测器在调制传递函数(MTF)和探测量子效率(DQE)方面比以往的探测器有了很大的改进。然而,这些新探测器的性能需要仔细监测,以便优化成像条件,并检查随着时间的推移是否退化。我们开发了一个简单易用的软件工具FindDQE,可以使用显微镜内置光束挡板的图像来测量电子探测器的MTF和DQE。使用该软件,我们已经测定了目前可用的四种直接电子探测器的DQE曲线:Gatan K2 Summit,FEI Falcon I和II,以及Direct Electron DE-12,在不同的总剂量和剂量率条件下。此外,我们还测量了Gatan US4000和TVIPS F416闪烁体相机的曲线。我们将新方法的结果与已发表的曲线进行了比较。
A new generation of direct electron detectors for transmission electron microscopy (TEM) promises significant improvement over previous detectors in terms of their modulation transfer function (MTF) and detective quantum efficiency (DQE). However, the performance of these new detectors needs to be carefully monitored in order to optimize imaging conditions and check for degradation over time. We have developed an easy-to-use software tool, FindDQE, to measure MTF and DQE of electron detectors using images of a microscope’s built-in beam stop. Using this software, we have determined the DQE curves of four direct electron detectors currently available: the Gatan K2 Summit, the FEI Falcon I and II, and the Direct Electron DE-12, under a variety of total dose and dose rate conditions. We have additionally measured the curves for the Gatan US4000 and TVIPS F416 scintillator-based cameras. We compare the results from our new method with published curves.
电子计数和束诱导运动校正可实现近原子分辨率的单粒子冷冻电镜。
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