Functional shifts in insect microRNA evolution.
Functional shifts in insect microRNA evolution.
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DOI：
10.1093/gbe/evq053
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发表时间：
2010
影响因子：
3.3
通讯作者：
Griffiths-Jones S
中科院分区：
文献类型：
--
作者：
Marco A;Hui JH;Ronshaugen M;Griffiths-Jones S
微小RNA（miRNA）是一种短的内源性RNA分子，在转录后水平调节基因表达，并已被证明在动物发育过程中发挥关键作用。因此，鉴定和比较后生动物物种中的miRNA对于我们理解身体计划的进化至关重要。我们通过对小RNA文库的深度测序，对赤拟谷盗Tribolium castaneum的203个miRNAs进行了表征。我们可以从一项研究中得出结论，赤拟谷盗的miRNA集比模式昆虫黑腹果蝇的miRNA集至少大15%（尽管后者进行了数十次高通量测序实验）。在昆虫中，miRNAs的出生率和死亡率都很高。在拟谷盗中只有三分之一的miRNA序列是保守的。至少18种拟谷盗属的miRNA在脊椎动物中是保守的，但在果蝇中丢失。在拟谷盗属和果蝇属之间保守的超过五分之一的miRNA表现出转录、基因组组织和加工模式的变化，这些变化导致预测的功能转变。例如，13%的保守miRNA表现出种子移位，我们描述了11%的正向对中的臂转换事件。这些变化从根本上改变了预测的靶点，因此改变了orthopa-miRNAs的功能。通常，赤拟谷盗属的miRNA比果蝇属的miRNA更能代表昆虫祖先，并且在脊椎动物中更保守。
MicroRNAs (miRNAs) are short endogenous RNA molecules that regulate gene expression at the posttranscriptional level and have been shown to play critical roles during animal development. The identification and comparison of miRNAs in metazoan species are therefore paramount for our understanding of the evolution of body plans. We have characterized 203 miRNAs from the red flour beetle Tribolium castaneum by deep sequencing of small RNA libraries. We can conclude, from a single study, that the Tribolium miRNA set is at least 15% larger than that in the model insect Drosophila melanogaster (despite tens of high-throughput sequencing experiments in the latter). The rate of birth and death of miRNAs is high in insects. Only one-third of the Tribolium miRNA sequences are conserved in D. melanogaster, and at least 18 Tribolium miRNAs are conserved in vertebrates but lost in Drosophila. More than one-fifth of miRNAs that are conserved between Tribolium and Drosophila exhibit changes in the transcription, genomic organization, and processing patterns that lead to predicted functional shifts. For example, 13% of conserved miRNAs exhibit seed shifting, and we describe arm-switching events in 11% of orthologous pairs. These shifts fundamentally change the predicted targets and therefore function of orthologous miRNAs. In general, Tribolium miRNAs are more representative of the insect ancestor than Drosophila miRNAs and are more conserved in vertebrates.
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影响因子：
1.8
作者：
HOFACKER, IL;FONTANA, W;SCHUSTER, P
通讯作者：
SCHUSTER, P
影响因子：
7
作者：
de Wit, Elzo;Linsen, Sam E. V.;Berezikov, Eugene
通讯作者：
Berezikov, Eugene
影响因子：
14.9
作者：
Altuvia Y;Landgraf P;Lithwick G;Elefant N;Pfeffer S;Aravin A;Brownstein MJ;Tuschl T;Margalit H
通讯作者：
Margalit H
Mirbase：MicroRNA基因组学的工具。
影响因子：
14.9
作者：
Griffiths-Jones, Sam;Saini, Harpreet Kaur;van Dongen, Stijn;Enright, Anton J.
通讯作者：
Enright, Anton J.
影响因子：
3
作者：
Guerra-Assunção JA;Enright AJ
通讯作者：
Enright AJ