CrvA and CrvB form a curvature-inducing module sufficient to induce cell-shape complexity in Gram-negative bacteria.
CrvA and CrvB form a curvature-inducing module sufficient to induce cell-shape complexity in Gram-negative bacteria.
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DOI：
10.1038/s41564-021-00924-w
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发表时间：
2021-07
影响因子：
28.3
通讯作者：
Gitai Z
中科院分区：
文献类型：
--
作者：
Martin NR;Blackman E;Bratton BP;Chase KJ;Bartlett TM;Gitai Z
细菌物种具有不同的细胞形状，使运动，定植和毒力。细胞壁决定了细菌的形状，主要由两个细胞因子引导的合成机器，延长体和分裂体构建。然而，产生复杂形状的机制，如霍乱弧菌的曲杆形状，是不完全确定的。以前的研究已经报道，种特异性调节的细胞色素引导的机器能够形成复杂的细菌形状，如细胞曲率和细胞附属物。与此相反，我们报告，CrvA和CrvB是足以诱导复杂的细胞形状自主的细胞骨架在霍乱弧菌。CrvAB模块的自主性还使其能够在革兰氏阴性物种大肠杆菌、铜绿假单胞菌、新月柄杆菌和根癌土壤杆菌中诱导弯曲。使用诱导型基因表达，定量显微镜，和生物化学，我们表明，CrvA和CrvB规避需要通过细胞骨架元素的图案，通过相互调节，形成一个不对称的本地化，周质结构，直接结合到细胞壁。这种周质结构的组装和分解使细胞形状发生动态变化。生物信息学表明，CrvA和CrvB可能是从一个单一的祖先杂交蛋白。使用霍乱弧菌中的融合实验，我们发现合成的CrvA/B杂合蛋白足以自身诱导曲率，但是两种不同蛋白CrvA和Crv B的表达促进更快的曲率诱导。我们的结论是，形态的复杂性可以出现独立的细胞形状规格的核心细胞色素引导的合成机器。
Bacterial species have diverse cell shapes that enable motility, colonization, and virulence. The cell wall defines bacterial shape and is primarily built by two cytoskeleton-guided synthesis machines, the elongasome and the divisome. However, the mechanisms producing complex shapes, like the curved-rod shape of Vibrio cholerae, are incompletely defined. Previous studies have reported that species-specific regulation of cytoskeleton-guided machines enables formation of complex bacterial shapes such as cell curvature and cellular appendages. In contrast, we report that CrvA and CrvB are sufficient to induce complex cell shape autonomously of the cytoskeleton in V. cholerae. The autonomy of the CrvAB module also enables it to induce curvature in the Gram-negative species Escherichia coli, Pseudomonas aeruginosa, Caulobacter crescentus, and Agrobacterium tumefaciens. Using inducible gene expression, quantitative microscopy, and biochemistry we show that CrvA and CrvB circumvent the need for patterning via cytoskeletal elements by regulating each other to form an asymmetrically-localized, periplasmic structure that directly binds to the cell wall. The assembly and disassembly of this periplasmic structure enables dynamic changes in cell shape. Bioinformatics indicate that CrvA and CrvB may have diverged from a single ancestral hybrid protein. Using fusion experiments in V. cholerae, we find that a synthetic CrvA/B hybrid protein is sufficient to induce curvature on its own, but that expression of two distinct proteins, CrvA and CrvB, promotes more rapid curvature induction. We conclude that morphological complexity can arise independently of cell shape specification by the core cytoskeleton-guided synthesis machines.
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影响因子：
10.5
作者：
Charbon, Godefroid;Cabeen, Matthew T.;Jacobs-Wagner, Christine
通讯作者：
Jacobs-Wagner, Christine
DOI：
10.1073/pnas.89.22.10915
发表时间：
1992-11-15
影响因子：
11.1
作者：
HENIKOFF, S;HENIKOFF, JG
通讯作者：
HENIKOFF, JG
影响因子：
3.1
作者：
Doerr, Tobias;Moell, Andrea;Waldor, Matthew K.
通讯作者：
Waldor, Matthew K.
影响因子：
3.6
作者：
Howell, Matthew;Aliashkevich, Alena;Brown, Pamela J. B.
通讯作者：
Brown, Pamela J. B.
影响因子：
64.5
作者：
Bartlett TM;Bratton BP;Duvshani A;Miguel A;Sheng Y;Martin NR;Nguyen JP;Persat A;Desmarais SM;VanNieuwenhze MS;Huang KC;Zhu J;Shaevitz JW;Gitai Z
通讯作者：
Gitai Z