Glycogen synthase kinase 3 beta positively regulates Notch signaling in vascular smooth muscle cells: role in cell proliferation and survival.
Glycogen synthase kinase 3 beta positively regulates Notch signaling in vascular smooth muscle cells: role in cell proliferation and survival.
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DOI：
10.1007/s00395-011-0189-5
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发表时间：
2011-09
影响因子：
9.5
通讯作者：
Cahill PA
中科院分区：
文献类型：
--
作者：
Guha S;Cullen JP;Morrow D;Colombo A;Lally C;Walls D;Redmond EM;Cahill PA
The role of glycogen synthase kinase 3 beta (GSK-3β) in modulating Notch control of vascular smooth muscle cell (vSMC) growth (proliferation and apoptosis) was examined in vitro under varying conditions of cyclic strain and validated in vivo following changes in medial tension and stress. Modulation of GSK-3β in vSMC following ectopic expression of constitutively active GSK-3β, siRNA knockdown and pharmacological inhibition with SB-216763 demonstrated that GSK-3β positively regulates Notch intracellular domain expression, CBF-1/RBP-Jκ transactivation and downstream target gene mRNA levels, while concomitantly promoting vSMC proliferation and inhibiting apoptosis. In contrast, inhibition of GSK-3β attenuated Notch signaling and decreased vSMC proliferation and survival. Exposure of vSMC to cyclic strain environments in vitro using both a Flexercell™ Tension system and a novel Sylgard™ phantom vessel following bare metal stent implantation revealed that cyclic strain inhibits GSK-3β activity independent of p42/p44 MAPK and p38 activation concomitant with reduced Notch signaling and decreased vSMC proliferation and survival. Exposure of vSMC to changes in medial strain microenvironments in vivo following carotid artery ligation revealed that enhanced GSK-3β activity was predominantly localized to medial and neointimal vSMC concomitant with increased Notch signaling, proliferating nuclear antigen and decreased Bax expression, respectively, as vascular remodeling progressed. GSK-3β is an important modulator of Notch signaling leading to altered vSMC cell growth where low strain/tension microenvironments prevail.
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影响因子：
5.6
作者：
Leeper, Nicholas J.;Raiesdana, Azad;Kojima, Yoko;Chun, Hyung J.;Azuma, Junya;Maegdefessel, Lars;Kundu, Ramendra K.;Quertermous, Thomas;Tsao, Philip S.;Spin, Joshua M.
通讯作者：
Spin, Joshua M.
DOI：
10.1161/01.atv.17.10.2238
发表时间：
1997-10-01
影响因子：
8.7
作者：
Kumar, A;Lindner, V
通讯作者：
Lindner, V
影响因子：
4.8
作者：
Liu, F;Schaphorst, KL;Garcia, JGN
通讯作者：
Garcia, JGN
影响因子：
5
作者：
Gao, Wei;Ferguson, Gail;Cahill, Paul A.
通讯作者：
Cahill, Paul A.
影响因子：
3.6
作者：
Gosens, Reinoud;Meurs, Herman;Schmidt, Martina
通讯作者：
Schmidt, Martina