Inter-observer and intra-observer differences in measuring body length: a test in the common lizard, Zootoca vivipara

Inter-observer and intra-observer differences in measuring body length: a test in the common lizard, Zootoca vivipara
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观察者间和观察者内测量体长的差异:对普通蜥蜴(Zootoca vivipara)的测试

DOI:
10.1163/156853811x601636
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发表时间:
2011
期刊:
影响因子:
1.6
通讯作者:
Böhme W.
Böhme W.
中科院分区:
生物学3区
文献类型:
--
作者:
Roitberg E. S;Orlova V. F;Kuranova V. N;Bulakhova N. A;Zinenko O. I;Ljubisavljevic K;Shamgunova R. R;Carretero M. A;Clasen A;Fokt M. ;Böhme W.

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摘要:鼻口长度(SVL)是蜥蜴和蛇的一种常规的全身尺寸测量,被广泛用于各种生态、进化和分类学研究。通常使用来自多个研究人员(观察者)的数据来分析SVL变化的趋势,但由于测量方案中观察者之间的差异而可能产生的混淆效应从未得到适当的检查。这项研究报告了11位爬行学家在测量相同欧亚普通蜥蜴标本时的观察者间偏差(21个成年标本由8个观察者检查,另外192个标本由2个观察者检查)。随着时间的推移(测量间隔1.5-15个月),还估计了观察者内部偏差。在绝大多数比较中,第一作者和另一个观察者之间的平均差异在- 1.0到+0.8 mm之间,或者在- 1.9到+1.6%之间,如果用样本SVL值的百分比表示。揭示了性别和研究样本对研究偏倚的一些不规则影响,并对其可能的原因进行了讨论。我们建议集中收集SVL和其他形态测量数据的研究人员考虑测试观察者内部和观察者之间的偏差,并建立可用于重新检查的标准子样本。
AbstractThe snout-vent length (SVL), a conventional measure of overall body size in lizards and snakes, is used in a wide variety of ecological, evolutionary, and taxonomical studies. Trends in SVL variation are often analysed using data from several researchers (observers), but possible confounding effects due to inter-observer differences in measurement protocols have never been appropriately examined. This study reports inter-observer biases between eleven herpetologists who measured the same specimens of the Eurasian common lizards (21 adult specimens were examined by eight observers and additional 192 specimens by two observers). Intra-observer bias over time (1.5-15 months between measuring sessions) was also estimated. In the vast majority of comparisons, mean difference between the first author and another observer varied from −1.0 to +0.8 mm, or from −1.9 to +1.6% if expressed as a percent of the specimen's SVL value. Some non-regular effects of sex and study sample on the studied bias were revealed, and their possible reasons are discussed. We are advising the researchers who intensively collect SVL and other morphometric data to consider testing intra-observer and inter-observer biases and to establish etalon samples available for re-examinations.
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