Metabonomic investigation of single and multiple strain Trypanosoma brucei brucei infections.
Metabonomic investigation of single and multiple strain Trypanosoma brucei brucei infections.
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DOI：
10.4269/ajtmh.2011.10-0402
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发表时间：
2011-01
期刊：
影响因子：
--
通讯作者：
Balmer O
中科院分区：
文献类型：
--
作者：
Li JV;Saric J;Wang Y;Utzinger J;Holmes E;Balmer O
尽管混合感染很常见，并可能产生重要的流行病学和进化后果，但探索多菌株感染的生化效应的研究仍然很少。我们使用基于1H核磁共振(核磁共振)谱的代谢图谱策略，研究了NMRI小鼠对布鲁氏锥虫单一(STIB777AE-Green1或STIB246BA-Red1)及其混合感染的代谢反应。所有布鲁氏结核杆菌感染都会在感染后第4天引起尿液生化成分的改变，特征是2-羟基异己酸酯、D-3-羟基丁酸酯、乳酸、4-羟基苯乙酸酯、苯丙酮酸和4-羟基苯丙酮酸浓度升高，而马尿酸水平下降。虽然在感染单株或双株布鲁氏结核杆菌的小鼠中观察到的代谢特征没有明显的差异，但与感染红色菌株或同时感染两种菌株的小鼠相比，感染绿色品系的小鼠的代谢反应较慢。丙酮酸、苯丙酮酸和马尿酸与寄生虫血症相关，这可能有助于监测对治疗干预的反应。
Although co-infections are common and can have important epidemiologic and evolutionary consequences, studies exploring biochemical effects of multiple-strain infections remain scarce. We studied metabolic responses of NMRI mice to Trypanosoma brucei brucei single (STIB777AE-Green1 or STIB246BA-Red1) and co-infections using a 1H nuclear magnetic resonance (NMR) spectroscopy-based metabolic profiling strategy. All T. b. brucei infections caused an alteration in urinary biochemical composition by day 4 postinfection, characterized by increased concentrations of 2-oxoisocaproate, D-3-hydroxybutyrate, lactate, 4-hydroxyphenylacetate, phenylpyruvate, and 4-hydroxyphenylpyruvate, and decreased levels of hippurate. Although there were no marked differences in metabolic signatures observed in the mouse infected with a single or dual strain of T. b. brucei, there was a slower metabolic response in mice infected with T. b. brucei green strain compared with mice infected with either the red strain or both strains concurrently. Pyruvate, phenylpyruvate, and hippurate were correlated with parasitemia, which might be useful in monitoring responses to therapeutic interventions.
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影响因子：
7.3
作者：
Chlopicki, S.;Swies, J.;Gebicki, J.
通讯作者：
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影响因子：
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作者：
Rijnders, BJA;Van Wijngaerden, E;Peetermans, WE
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Peetermans, WE
DOI：
10.1016/s0035-9203(99)90322-x
发表时间：
1999-02-01
影响因子：
2.2
作者：
Smith, T;Beck, HP;Tanner, M
通讯作者：
Tanner, M
影响因子：
4.8
作者：
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通讯作者：
Westerhoff, HV
DOI：
10.1111/j.1471-8286.2006.01297.x
发表时间：
2006-06
期刊：
MOLECULAR ECOLOGY NOTES
影响因子：
--
作者：
Balmer, Oliver;Palma, Christopher;Macleod, Annette;Caccone, Adalgisa
通讯作者：
Caccone, Adalgisa