Secreted single-stranded DNA is involved in the initial phase of biofilm formation by Neisseria gonorrhoeae.
Secreted single-stranded DNA is involved in the initial phase of biofilm formation by Neisseria gonorrhoeae.
复制标题
分泌的单链 DNA 参与淋病奈瑟菌生物膜形成的初始阶段
DOI：
10.1111/1462-2920.12291
复制
发表时间：
2014
影响因子：
5.1
通讯作者：
C van der Does
中科院分区：
文献类型：
--
作者：
Schork;Koerdt;Siewering;Sternberg;Thormann;Albers;C van der Does
Please try later.
Neisseria gonorrhoeaeis an obligate human pathogen that colonizes the genital tract and causes gonorrhoea.Neisseria gonorrhoeaecan form biofilms during natural cervical infections, on glass and in continuous flow‐chamber systems. These biofilms contain large amounts of extracellular DNA, which plays an important role in biofilm formation. Many clinical isolates contain a gonococcal genetic island that encodes a type IV secretion system (T4SS). The T4SS ofN. gonorrhoeaestrain MS11 secretes ssDNA directly into the medium. Biofilm formation, studied in continuous flow‐chamber systems by confocal laser scanning microscopy (CLSM), was strongly reduced, especially in the initial phases of biofilm formation, in the presence of Exonuclease I, which specifically degrades ssDNA or in a ΔtraBstrain that does not secrete ssDNA. To specifically detect ssDNA in biofilms using CLSM, a novel method was developed in which thermostable fluorescently labelled ssDNA‐ and ss/dsDNA‐binding proteins were used to visualize ssDNA and total DNA in biofilms and planktonic cultures. Remarkably, mainly dsDNA was detected in biofilms of the ssDNA secreting strain. We conclude that the secreted ssDNA facilitates initial biofilm formation, but that the secreted ssDNA is not retained in mature biofilms.
登录
查看更多内容
DOI：
--
发表时间：
--
期刊：
--
影响因子：
--
作者：
Gustavo Arrizabalaga;Carmen;Sinai
通讯作者：
Gustavo Arrizabalaga;Carmen;Sinai
铜绿假单胞菌KYU-1排泄DNA机制的研究
DOI：
10.1271/bbb1961.45.2457
发表时间：
1981
期刊：
Agricultural and biological chemistry
影响因子：
--
作者：
T. Hara;S. Ueda
通讯作者：
S. Ueda
一种完全明确的、透明的、无蛋白质的液体培养基，允许淋病奈瑟菌从极低的接种量中密集生长。
影响因子：
2.1
作者：
J. Wade;M. Graver
通讯作者：
M. Graver
影响因子：
3.6
作者：
O'Toole, GA;Kolter, R
通讯作者：
Kolter, R
DOI：
10.1080/00021369.1981.10864927
发表时间：
1981-01-01
期刊：
AGRICULTURAL AND BIOLOGICAL CHEMISTRY
影响因子：
--
作者：
HARA, T;UEDA, S
通讯作者：
UEDA, S