Single-cell technologies to decipher cardiovascular diseases.
Single-cell technologies to decipher cardiovascular diseases.
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DOI：
10.1093/eurheartj/ehac095
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发表时间：
2022-11-14
影响因子：
39.3
通讯作者：
Dimmeler, Stefanie
中科院分区：
文献类型：
--
作者：
Abplanalp, Wesley Tyler;Tucker, Nathan;Dimmeler, Stefanie
关键词：
心血管疾病仍然是全世界死亡的主要原因。对多细胞组成和分子过程的深入了解可能有助于确定新的治疗策略。单细胞技术，如单细胞或单核RNA测序，提供了单个细胞的表达谱，并允许在健康和疾病期间解剖组织中的异质性。本综述将总结（i）这些新技术如何成为描述心血管疾病（特别是人类）的机制驱动因素的关键，以及（ii）它们如何作为风险分层或个体化治疗的诊断工具。审查将进一步讨论技术缺陷，并概述可用作研究资源的公开的人类和小鼠数据集。心血管疾病研究组织的单细胞分析。利用scRNA或snRNA测序能够实现对组织异质性、转录应答和细胞通信的深入分析的机会。
Cardiovascular disease remains the leading cause of death worldwide. A deeper understanding of the multicellular composition and molecular processes may help to identify novel therapeutic strategies. Single-cell technologies such as single-cell or single-nuclei RNA sequencing provide expression profiles of individual cells and allow for dissection of heterogeneity in tissue during health and disease. This review will summarize (i) how these novel technologies have become critical for delineating mechanistic drivers of cardiovascular disease, particularly, in humans and (ii) how they might serve as diagnostic tools for risk stratification or individualized therapy. The review will further discuss technical pitfalls and provide an overview of publicly available human and mouse data sets that can be used as a resource for research. Single-cell analysis of tissues studied in cardiovascular disease. Opportunities for in-depth analysis of tissue heterogeneity, transcriptional responses, and cell communication are enabled with scRNA or snRNA sequencing.
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影响因子：
4.5
作者：
Fan J;Wang X;Xiao R;Li M
通讯作者：
Li M
影响因子：
10.8
作者：
Abplanalp, Wesley T.;John, David;Dimmeler, Stefanie
通讯作者：
Dimmeler, Stefanie
影响因子：
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Lundeberg, Joakim
单细胞染色质可及性揭示了调节变化的原理。
影响因子：
64.8
作者：
Buenrostro JD;Wu B;Litzenburger UM;Ruff D;Gonzales ML;Snyder MP;Chang HY;Greenleaf WJ
通讯作者：
Greenleaf WJ
影响因子：
48
作者：
Habib N;Avraham-Davidi I;Basu A;Burks T;Shekhar K;Hofree M;Choudhury SR;Aguet F;Gelfand E;Ardlie K;Weitz DA;Rozenblatt-Rosen O;Zhang F;Regev A
通讯作者：
Regev A