On the difference between surface-enhanced raman scattering (SERS) spectra of cell growth media and whole bacterial cells.
On the difference between surface-enhanced raman scattering (SERS) spectra of cell growth media and whole bacterial cells.
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DOI：
10.1366/10-06173
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发表时间：
2011-05
影响因子：
3.5
通讯作者：
Ziegler LD
中科院分区：
文献类型：
--
作者：
Premasiri WR;Gebregziabher Y;Ziegler LD
最近有人提出[N]。E. Marotta和L. A. Bottomley，应用光谱学。64，2010，601-06]，先前报道的营养细菌细胞的Sers光谱是由于没有从实验室培养的微生物悬浮液的样品中适当去除的残留细胞生长培养基。几种常用的细胞生长培养基的Sers光谱与细菌细胞的相似，如本文所示和其他地方报道的。然而，多变量数据分析方法表明，在相同的生长介质中生长的不同细菌物种的Sers光谱表现出不同的特征振动光谱，在不同的介质中生长的相同的生物体的Sers光谱显示相同的Sers光谱，并且生长介质的Sers光谱不聚集在洗涤细菌的Sers光谱附近。此外，在基本培养基中生长的细菌Sers光谱，其使用无机物作为氮源并且几乎不显示Sers特征，表现出特征性的细菌Sers光谱。我们使用多变量分析来显示连续的水洗和离心循环如何去除细胞生长培养基，并导致与先前将细菌Sers信号归因于生长培养基的研究相反的稳健的细菌Sers光谱。
It has been recently suggested [N. E. Marotta and L. A. Bottomley, Appl. Spectrosc. 64, 2010, 601-06] that previously reported SERS spectra of vegetative bacterial cells are due to residual cell growth media that were not properly removed from samples of the lab cultured microorganism suspensions. SERS spectra of several commonly used cell growth media are similar to those of bacterial cells as shown here and reported elsewhere. However, a multivariate data analysis approach shows that SERS spectra of different bacterial species grown in the same growth media exhibit different characteristic vibrational spectra, SERS spectra of the same organism grown in different media display the same SERS spectrum, and SERS spectra of growth media do not cluster near the SERS spectra of washed bacteria. Furthermore, a bacterial SERS spectrum grown in a minimal medium, which uses inorganics for a nitrogen source and displays virtually no SERS features, exhibits a characteristic bacterial SERS spectrum. We use multivariate analysis to show how successive water washing and centrifugation cycles remove cell growth media and result in a robust bacterial SERS spectrum in contrast to the previous study attributing bacterial SERS signals to growth media.
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影响因子：
3.5
作者：
Marotta, Nicole E.;Bottomley, Lawrence A.
通讯作者：
Bottomley, Lawrence A.
影响因子：
2.5
作者：
Guicheteau, Jason;Christesen, Steven;Tripathi, Ashish
通讯作者：
Tripathi, Ashish
影响因子：
7.4
作者：
Jarvis, RM;Goodacre, R
通讯作者：
Goodacre, R
影响因子：
3.3
作者：
Premasiri, WR;Moir, DT;Ziegler, LD
通讯作者：
Ziegler, LD
影响因子：
3.5
作者：
Zeiri, L;Bronk, BV;Efrima, S
通讯作者：
Efrima, S