Prevalence of anti-leptospiral IgM and detection of pathogenic Leptospira species DNA in neonates presenting with clinical sepsis in Southwestern Uganda.
Prevalence of anti-leptospiral IgM and detection of pathogenic Leptospira species DNA in neonates presenting with clinical sepsis in Southwestern Uganda.
复制标题
DOI：
10.1186/s40001-022-00902-w
复制
发表时间：
2022-12-02
影响因子：
4.2
通讯作者：
Bazira, Joel
中科院分区：
文献类型：
--
作者：
Hope, Derick;Businge, Stephen;Kyoyagala, Stella;Bazira, Joel
钩端螺旋体病是一种新出现的被忽视的人畜共患病，表现出非特异性体征/症状，可能会被误认为是其他疾病。由于诊断能力有限和认识不足，许多撒哈拉以南国家关于人类钩端螺旋体病、特别是新生儿钩端螺旋体病的数据很少。报道不足，阻碍了预防和控制措施的落实。该研究旨在使用 IgM ELISA 和实时定量 PCR (qPCR) 确定钩端螺旋体病作为新生儿发热性疾病原因的患病率。这是一项描述性横断面研究，纳入了 103 例新生儿败血症病例，其父母/法定监护人均给予知情同意。使用结构化数据收集表格收集人口统计和临床特征的数据。由训练有素的研究护士从新生儿采集 EDTA 全血样本。使用自动分析仪对样本进行 IgM ELISA，提取 DNA，并使用针对致病性钩端螺旋体特异的 LipL32 引物进行 qPCR。 ELISA测定新生儿抗钩端螺旋体IgM患病率为4.3%，均表现为嗜睡、喂养不良。 qPCR 未扩增出致病性钩端螺旋体 DNA。本研究在新生儿败血症病例中证实了钩端螺旋体病的证据。研究结果表明，在脓毒症新生儿的鉴别诊断中应考虑钩端螺旋体病。需要更多关于新生儿钩端螺旋体病的真实流行病学、临床特征和负担的数据。诊断性 qPCR 检测中需要包括钩端螺旋体的中间致病菌种。在线版本包含可在 10.1186/s40001-022-00902-w 获取的补充材料。
Leptospirosis is an emerging neglected zoonotic disease that presents with nonspecific signs/symptoms and it can be mistaken for other diseases. Owing to limited diagnostic capacity and unawareness, the data on human leptospirosis particularly in neonates are scarce in many sub-Saharan countries. It has been underreported hindering preventive and control measures in place. The study aimed at determining prevalence of leptospirosis as a cause of febrile illness in neonates using IgM ELISA and a quantitative real-time PCR (qPCR). This was a descriptive cross-sectional study that included 103 neonatal sepsis cases whose parents/legal guardians gave informed consent. The data on demographic and clinical characteristics were collected using structured data collection form. EDTA whole blood sample was collected from the neonates by trained study nurses. From the samples, IgM ELISA was done using automated analyzers, DNA extracted and qPCR was performed using primers for LipL32, specific for the pathogenic leptospires. The prevalence of anti-leptospiral IgM among the neonates as determined by ELISA was 4.3%, where all of them presented with lethargy and poor feeding. No pathogenic Leptospira species DNA was amplified by qPCR. Evidence of leptospirosis was demonstrated in neonatal sepsis cases in this study. The findings suggest considerations of leptospirosis in the differential diagnosis of neonates with sepsis. More data are needed on the real epidemiology, clinical features, and burden of leptospirosis in neonates. There is need to include intermediate pathogenic species of Leptospira in the diagnostic qPCR assays. The online version contains supplementary material available at 10.1186/s40001-022-00902-w.
登录
查看更多内容
动物和人类钩端螺旋体分离物的特征：系统发育分析确定了印度中间物种的流行。
影响因子：
--
作者：
Balamurugan V;Gangadhar NL;Mohandoss N;Thirumalesh SR;Dhar M;Shome R;Krishnamoorthy P;Prabhudas K;Rahman H
通讯作者：
Rahman H
影响因子：
3.8
作者：
Dreyfus A;Dyal JW;Pearson R;Kankya C;Kajura C;Alinaitwe L;Kakooza S;Pelican KM;Travis DA;Mahero M;Boulware DR;Mugisha L
通讯作者：
Mugisha L
影响因子：
3.9
作者：
Schiff, Steven J.;Kiwanuka, Julius;Poss, Mary
通讯作者：
Poss, Mary
影响因子：
3.7
作者：
Kiwanuka, Julius;Bazira, Joel;Schiff, Steven J.
通讯作者：
Schiff, Steven J.
影响因子：
3.8
作者：
Vincent, Antony T.;Schiettekatte, Olivier;Picardeau, Mathieu
通讯作者：
Picardeau, Mathieu