Characterization of leptospira isolates from animals and humans: phylogenetic analysis identifies the prevalence of intermediate species in India.
Characterization of leptospira isolates from animals and humans: phylogenetic analysis identifies the prevalence of intermediate species in India.
复制标题
动物和人类钩端螺旋体分离物的特征：系统发育分析确定了印度中间物种的流行。
DOI：
10.1186/2193-1801-2-362
复制
发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Rahman H
中科院分区：
文献类型：
--
作者：
Balamurugan V;Gangadhar NL;Mohandoss N;Thirumalesh SR;Dhar M;Shome R;Krishnamoorthy P;Prabhudas K;Rahman H
本研究在Ellinghausen McCullough Johnson and Harris(EMJH)培养基中从疑似动物和人的标本中回收191个培养菌株，并用暗视野显微镜对其形态特征进行鉴定。从单个培养物中提取的DNA进行不同的PCR方法进行钩端螺旋体的鉴定和鉴定。在99株阳性钩端螺旋体培养物中，52株致病性钩端螺旋体分离株通过部分核糖核酸聚合酶β亚单位(RpoB)基因序列在种水平上进行了鉴定。核苷酸序列的系统发育分析表明，30株、8株和14株分别属于博氏钩端螺旋体/问号钩端螺旋体、克氏钩端螺旋体和钩端螺旋体中间种。通过对99株钩端螺旋体的分析，在动物和人中流行的钩端螺旋体是伯氏钩端螺旋体或问号钩端螺旋体(30.30%)、克氏钩端螺旋体(8%)和中间钩端螺旋体(14.14%)。据作者所知，这是首次在印度利用基于rpoB基因核苷酸序列的系统发育分析来鉴定/检测动物和人类中的中间钩端螺旋体(L.wolffii)。因此，这一物种的流行肯定会强调考虑中间钩端螺旋体的重要性，并为进一步研究，特别是在了解动物和人类中新出现的钩端螺旋体，以及应对与疾病条件相关的问题奠定基础。
In this study, 191 culture isolates were recovered from suspected samples of animals and humans in Ellinghausen McCullough Johnson and Harris (EMJH) medium and assessed for its morphological features by dark field microscopy. Extracted DNA from individual culture was subjected to different PCR assays for identification and characterization of leptospira. Out of 99 positive leptospira cultures, 52 pathogenic leptospira isolates were characterized at species level by using partial RNA polymerase β-subunit (rpoB) gene sequences. Phylogenetic analysis of the nucleotide sequences revealed that 30, 8, and 14 isolates belong to L. borgpetersenii / L. interrogans, L. kirschneri, and Leptospira intermediate species, respectively. Based on analysis of 99 leptospira isolates, the prevalent Leptospira species were L. borgpetersenii or L. interrogans (30.30%), L. kirschneri (8%) and Leptospira intermediate species (14.14%) in animals and humans. To the best of authors knowledge, this is the first study to use rpoB gene nucleotide sequence based phylogenetic analysis to identify/detect Leptospira intermediate species (L. wolffii) in animals and humans in India. Hence, the prevalence of this species will surely emphasize the importance of consideration of Leptospira intermediate species and formulate a way for further studies especially in understanding the newly emerging Leptospira in animals and humans and to combat the problem associated with the disease conditions.
登录
查看更多内容
影响因子：
9.4
作者：
Faber, NA;Crawford, M;Willits, NH
通讯作者：
Willits, NH
DOI：
10.20506/rst.27.3.1847
发表时间：
2008-12-01
影响因子：
0.6
作者：
Gangadhar, N. L.;Prabhudas, K.;Rehaman, H.
通讯作者：
Rehaman, H.
DOI：
10.1111/j.1439-0450.2004.00740.x
发表时间：
2004-04-01
期刊：
JOURNAL OF VETERINARY MEDICINE SERIES B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH
影响因子：
--
作者：
Hartskeerl, RA;Goris, MGA;Wollanke, B
通讯作者：
Wollanke, B
影响因子：
3.2
作者：
PASTER, BJ;DEWHIRST, FE;WOESE, CR
通讯作者：
WOESE, CR
DOI：
10.1099/ijs.0.63783-0
发表时间：
2006-03-01
影响因子：
2.8
作者：
Levett, PN;Morey, RE;Steigerwalt, AG
通讯作者：
Steigerwalt, AG