Salmonella enterica serotype Typhimurium Std fimbriae bind terminal alpha(1,2)fucose residues in the cecal mucosa.
Salmonella enterica serotype Typhimurium Std fimbriae bind terminal alpha(1,2)fucose residues in the cecal mucosa.
复制标题
DOI：
10.1111/j.1365-2958.2008.06566.x
复制
发表时间：
2009-02
影响因子：
3.6
通讯作者：
Bäumler AJ
中科院分区：
文献类型：
--
作者：
Chessa D;Winter MG;Jakomin M;Bäumler AJ
标准操纵子编码鼠伤寒沙门氏菌血清型的菌毛粘附素，其是附着于肠上皮细胞和在小鼠盲肠定植所必需的。为了研究这种毒力因子促进定植的机制，我们表征了其结合特异性。通过去除 N 连接聚糖，可以消除标准介导的与人结肠上皮 (Caco-2) 细胞的结合。通过与 H 型 2 寡糖 (Fucα1-2Galβ1-4GlcNAc) 共孵育或用 α1-2 岩藻糖苷酶预处理细胞，可以阻断标准流苏鼠伤寒沙门氏菌对 Caco-2 细胞的粘附。相比之下，用神经氨酸酶预处理 Caco-2 细胞或与 2 型二糖前体 (Galβ1-4GlcNAc) 共孵育不会降低 Std 流苏鼠伤寒沙门氏菌的粘附。在固相结合测定中，纯化的 Std 菌毛与 Fucα1-2Galβ1-4GlcNAc 的结合被 Ulex europaeus 凝集素-I (UEA-I)（一种对 Fucα1-2 部分具有特异性的凝集素）竞争性抑制。纯化的标准菌毛和 UEA 均与位于小鼠盲肠粘液层的受体结合。这些数据表明，std操纵子编码一种粘附素，该粘附素与盲肠粘膜中存在的α1-2岩藻糖基化受体结合。
The std operon encodes a fimbrial adhesin of Salmonella enterica serotype Typhimurium that is required for attachment to intestinal epithelial cells and for cecal colonization in the mouse. To study the mechanism by which this virulence factor contributes to colonization we characterized its binding specificity. Std-mediated binding to human colonic epithelial (Caco-2) cells could be abrogated by removing N-linked glycans. Adherence of Std fimbriated S. Typhimurium to Caco-2 cells could be blocked by co-incubation with H type 2 oligosaccharide (Fucα1-2Galβ1-4GlcNAc) or by pretreatment of cells with α1-2 fucosidase. In contrast, pretreatment of Caco-2 cells with neuraminidase or co-incubation with the type 2 disaccharide precursor (Galβ1-4GlcNAc) did not reduce adherence of Std fimbriated S. Typhimurium. Binding of purified Std fimbriae to Fucα1-2Galβ1-4GlcNAc in a solid phase binding assay was competitively inhibited by Ulex europaeus agglutinin-I (UEA-I), a lectin specific for Fucα1-2 moieties. Purified Std fimbriae and UEA both bound to a receptor localized in the mucus layer of the murine cecum. These data suggest that the std operon encodes an adhesin that binds an α1-2 fucosylated receptor(s) present in the cecal mucosa.
登录
查看更多内容
影响因子：
7.3
作者：
Azevedo, M.;Eriksson, S.;David, L.
通讯作者：
David, L.
影响因子：
2
作者：
Chuang, Yin-Ching;Yang, Chia-Huei;Yeh, Kuang-Sheng
通讯作者：
Yeh, Kuang-Sheng
影响因子：
3.2
作者：
Porwollik, S;Boyd, EF;McClelland, M
通讯作者：
McClelland, M
影响因子：
3.2
作者：
Chan, K;Baker, S;Falkow, S
通讯作者：
Falkow, S
影响因子：
3.2
作者：
Balbontin, Roberto;Rowley, Gary;Casadesus, Josep
通讯作者：
Casadesus, Josep