GPT2: a glucose 6-phosphate/phosphate translocator with a novel role in the regulation of sugar signalling during seedling development.
GPT2: a glucose 6-phosphate/phosphate translocator with a novel role in the regulation of sugar signalling during seedling development.
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DOI：
10.1093/aob/mct298
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发表时间：
2014-03
期刊：
影响因子：
4.2
通讯作者：
Johnson GN
中科院分区：
文献类型：
--
作者：
Dyson BC;Webster RE;Johnson GN
GPT 2是一种葡萄糖6-磷酸/磷酸转运蛋白，在拟南芥成熟叶片的环境感知中起着重要作用。它的表达也在拟南芥种子和幼苗中检测到。为了研究这种蛋白在发育早期的作用，研究了萌发和幼苗生长。在野生型拟南芥和gpt 2 T-DNA插入敲除系中监测发芽、绿化和幼苗建立。在存在或不存在葡萄糖和脱落酸的情况下，将种子播种在琼脂平板上。使用定量PCR测量幼苗中GPT 2的相对表达。缺乏GPT 2表达的植物在幼苗建立中被延迟（25- 40%），特别是在子叶绿化过程中（而不是发芽）。这种表型不能通过生长培养基中的葡萄糖来拯救，其中绿化对葡萄糖高度敏感。发芽本身，然而，在gpt 2突变体的葡萄糖低敏感性。GPT 2的表达调节幼苗发育，并在确定幼苗建立过程中对外源糖的反应中起着至关重要的作用。这使我们能够得出结论，内源性糖信号的功能，在控制发芽和过渡，从异养到自养生长，和葡萄糖6-磷酸，或相关代谢产物的分配，细胞质和质体之间调节这些发育反应。
GPT2, a glucose 6-phosphate/phosphate translocator, plays an important role in environmental sensing in mature leaves of Arabidopsis thaliana. Its expression has also been detected in arabidopsis seeds and seedlings. In order to examine the role of this protein early in development, germination and seedling growth were studied. Germination, greening and establishment of seedlings were monitored in both wild-type Arabidopsis thaliana and in a gpt2 T-DNA insertion knockout line. Seeds were sown on agar plates in the presence or absence of glucose and abscisic acid. Relative expression of GPT2 in seedlings was measured using quantitative PCR. Plants lacking GPT2 expression were delayed (25–40 %) in seedling establishment, specifically in the process of cotyledon greening (rather than germination). This phenotype could not be rescued by glucose in the growth medium, with greening being hypersensitive to glucose. Germination itself was, however, hyposensitive to glucose in the gpt2 mutant. The expression of GPT2 modulates seedling development and plays a crucial role in determining the response of seedlings to exogenous sugars during their establishment. This allows us to conclude that endogenous sugar signals function in controlling germination and the transition from heterotrophic to autotrophic growth, and that the partitioning of glucose 6-phosphate, or related metabolites, between the cytosol and the plastid modulates these developmental responses.
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影响因子：
64.5
作者：
DENG, XW;GRUISSEM, W
通讯作者：
GRUISSEM, W
影响因子：
7.4
作者：
Athanasiou, Kleovoulos;Dyson, Beth C.;Johnson, Giles N.
通讯作者：
Johnson, Giles N.
影响因子：
4.3
作者：
Corbesier, L;Lejeune, P;Bernier, G
通讯作者：
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影响因子：
7.4
作者：
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通讯作者：
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影响因子：
7.2
作者：
FINKELSTEIN, RR
通讯作者：
FINKELSTEIN, RR