Antidiarrheal, antimicrobial and antioxidant potentials of methanol extract of Colocasia gigantea Hook. f. leaves: evidenced from in vivo and in vitro studies along with computer-aided approaches.
Antidiarrheal, antimicrobial and antioxidant potentials of methanol extract of Colocasia gigantea Hook. f. leaves: evidenced from in vivo and in vitro studies along with computer-aided approaches.
复制标题
DOI：
10.1186/s12906-021-03290-6
复制
发表时间：
2021-04-12
影响因子：
3.9
通讯作者：
Haque MR
中科院分区：
文献类型：
--
作者：
Alam S;Rashid MA;Sarker MMR;Emon NU;Arman M;Mohamed IN;Haque MR
芋茶，当地称为kochu是众所周知的，由于其各种愈合能力。本研究旨在探讨芋芋甲醇提取物的抗真菌、抑菌及抗氧化活性。采用蓖麻油诱导法进行体内抗菌活性研究，采用纸片扩散法和DPPH自由基清除法进行体外抗菌活性和抗氧化活性研究。此外，在计算机模拟研究之后，对使用Schrödinger-Maestro v11.1和Biovia Discovery Studio进行评价的几种次级代谢产物进行了分子对接分析。与对照组相比，植物提取物（200和400 mg/kg，B.w，p.o）的诱导使蓖麻油介导的腹泻分别最小化16.96%（p < 0.01）和38.89%（p < 0.001）。C.巨茶对几乎所有供试菌株均表现出轻度敏感性，但酚类含量一致性较高，DPPH测试IC 50值为67.68 μg/mL。在PASS预测中，选择的分离的化合物已经证明了显著的抗真菌和抗微生物活性，遵循Lipinski药物规则，该规则已经确定了化合物在分子对接研究中的功效。本研究结果反映了川楝子的甲醇提取物具有较好的生物学活性。绿茶是安全的，可以提供缓解腹泻的可能性，沿着是抗氧化剂和抗菌剂的潜在源泉，可以被认为是在不久的将来开发新药品的替代来源。
Colocasia gigantea, locally named as kochu is well-known due to its various healing power. This research is to investigate the antidiarrheal, antimicrobial and antioxidant possibilities of the methanol soluble extract of Colocasia gigantea. The antidiarrheal investigation was performed by using in vivo castor oil-induced diarrheal method whereas in vitro antimicrobial and antioxidant investigation have been implemented by disc diffusion and DPPH scavenging method respectively. Moreover, in silico studies were followed by molecular docking analysis of several secondary metabolites that were appraised with Schrödinger-Maestro v11.1 and Biovia Discovery Studio. The induction of plant extract (200 and 400 mg/kg, b.w, p.o) has minimized the castor oil mediated diarrhea by 16.96% (p < 0.01) and 38.89% (p < 0.001) respectively compared to control group. The methanol extract of C. gigantea showed mild sensitivity against almost all the tested strains but it shows high consistency of phenolic content and yielded 67.68 μg/mL of IC50 value in the DPPH test. In the PASS prediction, selected isolated compounds have demonstrated significant antidiarrheal and antimicrobial activity following the Lipinski drug rules which have ascertained efficacy with the compounds in molecular docking study. The results of this scientific research reflects that the methanol soluble extract of C. gigantea is safe and may provide possibilities of alleviation of diarrhea along with being a potential wellspring of antioxidant and antimicrobial agents which can be considered as an alternate source for exploration of new medicinal products in near future.
登录
查看更多内容
选定的可食用植物提取物针对多药抗革兰氏阴性细菌的抗菌活性。
影响因子：
--
作者：
Djeussi DE;Noumedem JA;Seukep JA;Fankam AG;Voukeng IK;Tankeo SB;Nkuete AH;Kuete V
通讯作者：
Kuete V
影响因子：
16.6
作者：
Che, Tao;English, Justin;Roth, Bryan L.
通讯作者：
Roth, Bryan L.
DOI：
10.3390/molecules18066852
发表时间：
2013-06-10
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Blainski A;Lopes GC;de Mello JC
通讯作者：
de Mello JC
影响因子：
2.8
作者：
Ivancic, Anton;Roupsard, Olivier;Lebot, Vincent
通讯作者：
Lebot, Vincent
影响因子：
--
作者：
Davis, Judith A
通讯作者：
Davis, Judith A