Amelogenin-assisted ex vivo remineralization of human enamel: Effects of supersaturation degree and fluoride concentration.
Amelogenin-assisted ex vivo remineralization of human enamel: Effects of supersaturation degree and fluoride concentration.
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DOI：
10.1016/j.actbio.2011.01.028
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发表时间：
2011-05
影响因子：
9.7
通讯作者：
Xu, Xiaoming
中科院分区：
文献类型：
--
作者：
Fan, Yuwei;Nelson, James R.;Alvarez, Jason R.;Hagan, Joseph;Berrier, Allison;Xu, Xiaoming
形成类似牙釉质的有组织纳米晶体是牙釉质再矿化成功的关键。钙、磷酸盐、氟化物离子和淀粉原蛋白是体外组织羟基磷灰石（HAP）晶体形成的重要成分。然而，这些再矿化剂对釉质晶体形态的影响尚未得到深入的研究。本研究旨在探讨氟离子、过饱和度和淀粉原蛋白对离体再矿化人牙釉质晶体形态和组织的影响。将取出的第三磨牙切成薄片，进行酸蚀，使牙釉质表面浸泡在不同的再矿化溶液中。采用FE-SEM、ATR-FTIR和XRD分析再矿化牙釉质表面的晶体形态和矿物相。氟化物浓度和羟基磷灰石过饱和程度对晶体形态和组织有显著影响，晶体形态和组织由片状松散晶体变为棒状密集排列的纳米晶体阵列。在氟化物1.5±0.5 mg/L，淀粉原素40±10µg/mL， pH 6.8±0.4的条件下，观察到密集排列的氟化羟基磷灰石纳米棒。相图总结了形成致密或松散羟基磷灰石纳米晶体结构的条件。本研究为开发治疗龋病的新型牙材料提供了基础。
The formation of organized nanocrystals that resemble enamel is crucial for successful enamel remineralization. Calcium, phosphate and fluoride ions and amelogenin are important ingredients for the formation of organized hydroxyapatite (HAP) crystals in vitro. However, the effects of these remineralization agents on the enamel crystal morphology have not been thoroughly studied. The objective of this study was to investigate the effects of fluoride ions, supersaturation degree and amelogenin on the crystal morphology and organization of ex vivo remineralized human enamel. Extracted third molars were sliced thin and acid-etched to provide the enamel surface for immersion in different remineralization solutions. The crystal morphology and mineral phase of the remineralized enamel surface were analyzed by FE-SEM, ATR-FTIR and XRD. The concentration of fluoride and supersaturation degree of hydroxyapatite had significant effects on the crystal morphology and crystal organization, which varied from plate-like loose crystals to rod-like densely packed nanocrystal arrays. Densely packed arrays of fluoridated hydroxyapatite nanorods were observed under the following conditions: σ(HAP) = 10.2±2.0 with fluoride 1.5±0.5 mg/L and amelogenin 40±10 µg/mL, pH 6.8±0.4. A phase diagram summarized the conditions that form dense or loose hydroxyapatite nanocrystal structures. This study provides the basis for the development of novel dental materials for caries management.
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影响因子：
62.1
作者：
Wang, Lijun;Nancollas, George H.
通讯作者：
Nancollas, George H.
影响因子：
3
作者：
Beniash, Elia;Metzler, Rebecca A.;Lam, Raymond S. K.;Gilbert, P. U. P. A.
通讯作者：
Gilbert, P. U. P. A.
影响因子：
14
作者：
Fan, Yuwei;Sun, Zhi;Moradian-Oldak, Janet
通讯作者：
Moradian-Oldak, Janet
影响因子：
2.7
作者：
Moradian-Oldak, J;Goldberg, M
通讯作者：
Goldberg, M
DOI：
10.1177/10454411920030010601
发表时间：
1992-01-01
期刊：
CRITICAL REVIEWS IN ORAL BIOLOGY & MEDICINE
影响因子：
--
作者：
JOHNSSON, MSA;NANCOLLAS, GH
通讯作者：
NANCOLLAS, GH