Fluorometric microplate-based dimethoate assay using CdSe/ZnS quantum dots coated with a molecularly imprinted polymer
Fluorometric microplate-based dimethoate assay using CdSe/ZnS quantum dots coated with a molecularly imprinted polymer
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使用涂有分子印迹聚合物的 CdSe/ZnS 量子点进行基于荧光微孔板的乐果测定
DOI：
10.1007/s00604-019-3649-5
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发表时间：
2019-07
影响因子：
5.7
通讯作者：
Sun Xiaotao
中科院分区：
文献类型：
--
作者：
Yang Yukun;Chang Yuanyuan;Guo Yuanyuan;Yu Ligang;Zhang Guohua;Zhai Doudou;Wang Xiaomin;Sun Xiaotao
本文介绍了一种灵敏、选择性测定农药乐果的微板荧光分析法。采用分子印迹聚合物（MIP）包覆的CdSe/ZnS量子点（QDs）作为分子识别探针。在疏水性CdSe/ZnS量子点存在下，采用一步反相微乳液法合成了MIP包覆的量子点。以3-氨丙基三乙氧基硅烷为功能单体，正硅酸乙酯为交联剂，乐果为模板剂，进行共聚反应。当乐果在分子印迹聚合物的印迹位点上反弹时，包覆的量子点的荧光被乐果猝灭。在最佳条件下，荧光（最佳测量激发/发射波长为380/620 nm）在5.0-150 μg L− 1乐果浓度范围内线性下降，检测限为2.1 μg L−1（S/N= 3）。该方法用于真实的样品中乐果的测定。回收率为89.8%~ 98.0%，相对标准偏差小于4.9%。该方法快速、经济、灵敏、选择性好。使用的微孔板允许大量的samples.Graphical abstractionSchematic表示的灵敏度和选择性的荧光微孔板为基础的测定乐果（DM）的高通量识别的分子印迹聚合物（MIP）的基础上，涂布的CdSe/ZnS量子点（QD）的测定。DM对量子点的荧光有猝灭作用。
A microplate-based assay is described for the sensitive and selective fluorometric determination of the pesticide dimethoate. Molecularly imprinted polymer (MIP)-coated CdSe/ZnS quantum dots (QDs) are used as the molecular recognition probe. The MIP-coated QDs were synthesized using one-step reversed-phase microemulsion in the presence of hydrophobic CdSe/ZnS QDs. Copolymerization was performed by using 3-aminopropyltriethoxysilane as the functional monomer, tetraethoxysilane as the cross-linker, and dimethoate as the template. The fluorescence of the coated QDs is quenched by dimethoate when dimethoate becomes rebound on the imprinting sites of the MIP. Under the optimal conditions, fluorescence (best measured at excitation/emisison wavelengths of 380/620 nm) drops linearly in the 5.0–150 μg L−1dimethoate concentration range, and the limit of detection is 2.1 μg L−1(at S/N= 3). The assay was utilized for dimethoate determination in spiked real samples. Satisfactory recoveries (89.8%–98.0%) with relatively standard deviations of <4.9% are obtained. The method is rapid, cost-effective, sensitive, and selective. The use of microplate allows for the quantitation of a large number of samples simultaneously.Graphical abstractSchematic representation of sensitive and selective fluorometric microplate-based assay for the high-throughput determination of dimethoate (DM) based on recognition of molecularly imprinted polymer (MIP)-coated CdSe/ZnS quantum dots (QDs). DM exerts a quenching effect on the fluorescence of the QDs.
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影响因子：
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影响因子：
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