Reconstruction of neocortex: Organelles, compartments, cells, circuits, and activity.
Reconstruction of neocortex: Organelles, compartments, cells, circuits, and activity.
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新皮质的重建：细胞器，隔间，细胞，电路和活动。
DOI：
10.1016/j.cell.2022.01.023
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发表时间：
2022-03-17
期刊：
影响因子：
64.5
通讯作者：
Seung, H. Sebastian
中科院分区：
文献类型：
--
作者：
Turner, Nicholas L.;Macrina, Thomas;Bae, J. Alexander;Yang, Runzhe;Wilson, Alyssa M.;Schneider-Mizell, Casey;Lee, Kisuk;Lu, Ran;Wu, Jingpeng;Bodor, Agnes L.;Bleckert, Adam A.;Brittain, Derrick;Froudarakis, Emmanouil;Dorkenwald, Sven;Collman, Forrest;Kemnitz, Nico;Ih, Dodam;Silversmith, William M.;Zung, Jonathan;Zlateski, Aleksandar;Tartavull, Ignacio;Yu, Szi-chieh;Popovych, Sergiy;Mu, Shang;Wong, William;Jordan, Chris S.;Castro, Manuel;Buchanan, JoAnn;Bumbarger, Daniel J.;Takeno, Marc;Torres, Russel;Mahalingam, Gayathri;Elabbady, Leila;Li, Yang;Cobos, Erick;Zhou, Pengcheng;Suckow, Shelby;Becker, Lynne;Paninski, Liam;Polleux, Franck;Reimer, Jacob;Tolias, Andreas S.;Reid, R. Clay;da Costa, Nuno Macarico;Seung, H. Sebastian
我们从~250×140×90 μm3的电子显微镜图像中组装了L2/3小鼠初级视皮层的半自动重建，包括锥体和非锥体神经元、星形胶质细胞、小胶质细胞、少突胶质细胞和前体细胞、周细胞、脉管系统、细胞核、线粒体和突触。包括锥体细胞的一个子集的视觉反应。这些数据是公开的，沿着有程序化和三维互动访问工具。简短的插图说明了在皮层电路和神经元细胞生物学的结构功能的潜在应用的广度。线粒体和突触组织的特点是从索马的路径长度的函数。金字塔连接基序频率预测准确使用随机图的配置模型。从附近细胞接收更多连接的锥体细胞表现出更强和更可靠的视觉反应。示例代码显示了数据访问和分析。
We assembled a semi-automated reconstruction of L2/3 mouse primary visual cortex from ~250×140×90 μm3 of electron microscopic images, including pyramidal and non-pyramidal neurons, astrocytes, microglia, oligodendrocytes and precursors, pericytes, vasculature, nuclei, mitochondria, and synapses. Visual responses of a subset of pyramidal cells are included. The data are publicly available, along with tools for programmatic and three-dimensional interactive access. Brief vignettes illustrate the breadth of potential applications relating structure to function in cortical circuits and neuronal cell biology. Mitochondria and synapse organization are characterized as a function of path length from the soma. Pyramidal connectivity motif frequencies are predicted accurately using a configuration model of random graphs. Pyramidal cells receiving more connections from nearby cells exhibit stronger and more reliable visual responses. Sample code shows data access and analysis.
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影响因子：
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通讯作者：
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