Super-resolution fluorescence imaging with single molecules.
Super-resolution fluorescence imaging with single molecules.
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DOI：
10.1016/j.sbi.2013.07.010
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发表时间：
2013-10
影响因子：
6.8
通讯作者：
Moerner WE
中科院分区：
文献类型：
--
作者：
Sahl SJ;Moerner WE
通过荧光以高空间精度光学检测、成像和定位单分子的能力使得新兴的超分辨率显微镜方法成为可能，这些方法克服了远场光聚焦光学器件长期存在的衍射障碍。这些方法在固定细胞和活细胞中均可实现 20-40 nm 或更好的空间分辨率，目前正在成为对细胞过程中的结构细节进行微创时空分析的强大工具，受益于分辨率的提高。这篇简短的回顾简要介绍了基本原理，然后总结了二维和三维 (3D) 多色技术和更快的延时方案的最新主要发展和应用示例。讨论了定量成像的前景 - 就其校正偶极子发射引起的系统定位误差的能力以及提供纳米级细胞域内分子拷贝数的准确计数的能力而言。
The ability to detect, image and localize single molecules optically with high spatial precision by their fluorescence enables an emergent class of super-resolution microscopy methods which have overcome the longstanding diffraction barrier for far-field light-focusing optics. Achieving spatial resolutions of 20–40 nm or better in both fixed and living cells, these methods are currently being established as powerful tools for minimally-invasive spatiotemporal analysis of structural details in cellular processes which benefit from enhanced resolution. Briefly covering the basic principles, this short review then summarizes key recent developments and application examples of two- and three-dimensional (3D) multi-color techniques and faster time-lapse schemes. The prospects for quantitative imaging – in terms of its ability to correct for dipole-emission-induced systematic localization errors and to provide accurate counts of molecular copy numbers within nanoscale cellular domains – are discussed.
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影响因子：
16.2
作者：
Dani, Adish;Huang, Bo;Bergan, Joseph;Dulac, Catherine;Zhuang, Xiaowei
通讯作者：
Zhuang, Xiaowei
影响因子：
48
作者：
Biteen, Julie S.;Thompson, Michael A.;Tselentis, Nicole K.;Bowman, Grant R.;Shapiro, Lucy;Moerner, W. E.
通讯作者：
Moerner, W. E.
影响因子：
48
作者：
Dempsey, Graham T.;Vaughan, Joshua C.;Chen, Kok Hao;Bates, Mark;Zhuang, Xiaowei
通讯作者：
Zhuang, Xiaowei
DOI：
10.1126/science.1222981
发表时间：
2012-07-13
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Berk V;Fong JC;Dempsey GT;Develioglu ON;Zhuang X;Liphardt J;Yildiz FH;Chu S
通讯作者：
Chu S
影响因子：
4
作者：
Badieirostami, Majid;Lew, Matthew D.;Moerner, W. E.
通讯作者：
Moerner, W. E.