SSOP typing of the Tenth International Histocompatibility Workshop reference cell lines for HLA-C alleles.
SSOP typing of the Tenth International Histocompatibility Workshop reference cell lines for HLA-C alleles.
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第十届国际组织相容性研讨会参考细胞系 HLA-C 等位基因的 SSOP 分型。
DOI：
10.1111/j.1399-0039.1994.tb02376.x
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Yang,SY
中科院分区：
文献类型：
--
作者：
Levine,JE;Yang,SY
HLA-C基因产物是HLA I类分子中了解最少的，因为与HLA-A和-B相比，它们在细胞表面上的表达水平较低。然而，最近的证据表明，HLA-C分子在引发T细胞应答和控制NK细胞识别方面具有功能能力。在大多数人群中，大约20%至50%的HLA-C等位基因类型为“空白”。为了更好地定义HLA-C等位基因，我们分析了来自第十届国际组织相容性研讨会的98个广泛表征的B细胞系。使用两组基因座特异性引物，从细胞组制备的DNA中实现外显子2和3的选择性HLA-C特异性DNA扩增。我们使用64个序列特异性寡核苷酸探针（SSOP）与外显子2和3中的可变位点互补以产生杂交模式。在这些模式中发现了25个等位基因，包括纯合细胞系中的7个新等位基因和杂合细胞系中的7个潜在的新等位基因。新的等位基因和已知的等位基因之间的差异通常很小。通过SSOP模式在Cw“空白”细胞中鉴定出五个主要基团。此外，HLA-B特异性和HLA-C等位基因之间的联系与以前的观察结果相似。本研究表明，SSOP分型是有效的，在纯合子分型细胞，但不是在杂合子细胞中识别新的等位基因。此外，DNA分型可以促进所有HLA-C等位基因的鉴定，包括那些血清学分型为空白的等位基因。HLA-C位点可能比以前认识到的更具多态性。
HLA‐C gene products are the most poorly understood of the HLA class I molecules because they express at low level on the cell surface compared to HLA‐A and ‐B. However, recent evidence shows that HLA‐C molecules are functionally competent in eliciting T‐cell responses and in controlling NK‐cell recognition. Approximately 20 to 50% of HLA‐C alleles type “blank” in most populations. To provide a better definition of the HLA‐C alleles, we analyzed 98 extensively characterized B‐cell lines from the 10th International Histocompatibility Workshop. Selective HLA‐C‐specific DNA amplification of exons 2 and 3 from DNA prepared from the cell panel was achieved with the use of two sets of locus‐specific primers. We used 64 sequence‐specific oligonucleotide probes (SSOPs) complementary to variable sites in exons 2 and 3 to generate hybridization patterns. Twenty‐five alleles were found among these patterns, including seven new alleles in the homozygous cell lines and seven potential new alleles in heterozygous cell lines. Differences between the new alleles and known alleles were generally small. Five major groups were identified in the Cw “blank” cells by the SSOP patterns. In addition, linkage between HLA‐B specificities and HLA‐C alleles was similar to previous observations. The present study demonstrated that SSOP typing was effective in identifying new alleles in homozygous typing cells but not in the heterozygous cells. Also, DNA typing can facilitate the identification of all HLA‐C alleles, including those that serologically type as blanks. The HLA‐C locus may be more polymorphic than was previously recognized.
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影响因子：
29.7
作者：
and A Townsend;H. Bodmer
通讯作者：
H. Bodmer
影响因子：
--
作者：
OH, SH;FLEISCHHAUER, K;YANG, SY
通讯作者：
YANG, SY
影响因子：
5.4
作者：
NEEFJES, JJ;PLOEGH, HL
通讯作者：
PLOEGH, HL
影响因子：
5.4
作者：
HOGQUIST, KA;GRANDEA, AG;BEVAN, MJ
通讯作者：
BEVAN, MJ
HLA I 类启动子的调控复合物表现出位点特异性保守性，且等位基因变异有限。
DOI：
--
发表时间：
1994
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Cereb,N;Yang,SY
通讯作者：
Yang,SY