A fijiviral nonstructural protein triggers cell death in plant and bacterial cells via its transmembrane domain.
A fijiviral nonstructural protein triggers cell death in plant and bacterial cells via its transmembrane domain.
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DOI：
10.1111/mpp.13277
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发表时间：
2023-01
影响因子：
4.9
通讯作者：
Zhang, Heng-Mu
中科院分区：
文献类型：
--
作者：
Yuan, Zhengjie;Geng, Yanfei;Dai, Yuanxing;Li, Jing;Lv, Mingfang;Liao, Qiansheng;Xie, Li;Zhang, Heng-Mu
南方水稻黑条矮缩病毒（SRBSDV;斐济病毒，呼肠孤病毒科）近年来已成为东亚谷物生产的威胁。我们以前的细胞病理学研究表明，SRBSDV诱导一个类似程序性细胞死亡的过程中感染的组织，导致独特的生长异常。然而，导致细胞死亡的病毒产物仍然未知。在这里，P9 - 2蛋白，而不是它的RNA，被证明诱导大肠杆菌和植物细胞中的细胞死亡时，无论是局部表达的瞬时表达载体或系统使用异源病毒。计算机预测和荧光分析表明，病毒的非结构蛋白是针对质膜（PM）和其亚细胞定位的进一步修改废除其诱导细胞死亡的能力，表明其PM定位所需的细胞死亡诱导。预计P9 - 2在其中央疏水结构域内含有两个跨膜螺旋。一系列的突变实验进一步表明，其中央跨膜疏水结构域是细胞死亡诱导的关键，其保守的F90，Y101，和L103氨基酸残基可以发挥协同作用，在维持其诱导细胞死亡的能力。它在其他斐济病毒中的同源物也诱导植物和细菌细胞的细胞死亡，这意味着斐济病毒的非结构蛋白可能通过靶向保守的细胞因子或通过高度保守的机制引发细胞死亡。斐济病毒P9 - 2蛋白而不是RNA显示通过靶向植物细胞中的质膜诱导细胞死亡，表明该蛋白质可能负责增生感染组织中的程序性细胞死亡样过程。
Southern rice black‐streaked dwarf virus (SRBSDV; Fijivirus, Reoviridae) has become a threat to cereal production in East Asia in recent years. Our previous cytopathologic studies have suggested that SRBSDV induces a process resembling programmed cell death in infected tissues that results in distinctive growth abnormalities. The viral product responsible for the cell death, however, remains unknown. Here P9‐2 protein, but not its RNA, was shown to induce cell death in Escherichia coli and plant cells when expressed either locally with a transient expression vector or systemically using a heterologous virus. Both computer prediction and fluorescent assays indicated that the viral nonstructural protein was targeted to the plasma membrane (PM) and further modification of its subcellular localization abolished its ability to induce cell death, indicating that its PM localization was required for the cell death induction. P9‐2 was predicted to harbour two transmembrane helices within its central hydrophobic domain. A series of mutation assays further showed that its central transmembrane hydrophobic domain was crucial for cell death induction and that its conserved F90, Y101, and L103 amino acid residues could play synergistic roles in maintaining its ability to induce cell death. Its homologues in other fijiviruses also induced cell death in plant and bacterial cells, implying that the fijiviral nonstructural protein may trigger cell death by targeting conserved cellular factors or via a highly conserved mechanism. Fijiviral P9‐2 protein but not RNA was shown to induce cell death by targeting the plasma membrane in plant cells, suggesting that this protein could be responsible for a programmed cell death‐like process in infected tissues with hyperplasia.
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影响因子：
16.6
作者：
Bi K;Scalschi L;Jaiswal N;Mengiste T;Fried R;Sanz AB;Arroyo J;Zhu W;Masrati G;Sharon A
通讯作者：
Sharon A
病毒诱导的小管：病毒粒子通过昆虫载体中肠上皮基底层快速传播的载体
影响因子：
5.4
作者：
Jia, Dongsheng;Mao, Qianzhuo;Wei, Taiyun
通讯作者：
Wei, Taiyun
DOI：
10.1073/pnas.96.14.7956
发表时间：
1999-07-06
影响因子：
11.1
作者：
Lacomme, C;Cruz, SS
通讯作者：
Cruz, SS
影响因子：
64.8
作者：
KALDERON, D;RICHARDSON, WD;SMITH, AE
通讯作者：
SMITH, AE
影响因子：
5.5
作者：
Guo, Xinrui;Zhong, Debin;Chen, Songbiao
通讯作者：
Chen, Songbiao