Platelet derived growth factor (PDGF) stimulates development of bovine embryos during the fourth cell cycle.-猫眼课题宝

血小板衍生生长因子 (PDGF) 在第四个细胞周期中刺激牛胚胎的发育。

DOI：

发表时间：

1992

期刊：

Development

影响因子：

4.6

通讯作者：

中科院分区：

生物学2区

文献类型：

作者：

R. C. Larson;G. Ignotz;W. B. Currie

体外产生的2细胞牛胚胎在补充有各种生长因子组合的无血清培养基中培养，以测试这些多肽因子能够发出植入前发育信号的假设。典型培养方法在8细胞期发生的发育停滞可能通过刺激胚胎基因组表达的生长因子依赖性机制来缓解，从而模拟在第四细胞周期（8至16细胞期）期间输卵管中体内发生的事件。随后，其他生长因子可能促进致密化和胚泡形成，这些过程通常发生在子宫内。生长因子对早期胚胎的影响进行了评估，使用相差显微镜监测进展到8-细胞阶段，完成和持续时间的第四个细胞周期，囊胚形成。血小板衍生生长因子（PDGF）促进发展超过16-细胞阶段，在所有实验中检查的2-细胞胚胎的39.1%。这些胚胎的第四个细胞周期的持续时间约为26小时。在16细胞期后的发育过程中，PDGF将胚胎囊胚化的比例从12.7%降低到5.8%;相反，转化生长因子α（TGF α）在相同的发育时间段内起作用，将胚胎囊胚化的比例从8.6%增加到40.6%。使用无血清培养基的这些结果表明，PDGF标志着第四细胞周期的完成。TGF α，也许是碱性成纤维细胞生长因子（bFGF），促进囊胚形成的16细胞胚胎在随后的培养。

In vitro produced, 2-cell bovine embryos were cultured in serum-free medium supplemented with various combinations of growth factors to test the hypothesis that these polypeptide factors are able to signal preimplantation development. The developmental arrest that occurs during the 8-cell stage with typical culture methods might be relieved by a growth factor-dependent mechanism that would stimulate expression of the embryonic genome, thereby mimicking events that occur in vivo in the oviduct during the fourth cell cycle (8- to 16-cell stage). Subsequently, other growth factors might promote compaction and blastulation, processes which normally occur in the uterus. The effects of growth factors on early embryos were evaluated using phase contrast microscopy to monitor progression to the 8-cell stage, completion and duration of the fourth cell cycle, and blastocyst formation. Platelet derived growth factor (PDGF) promoted development beyond the 16-cell stage in 39.1% of the 2-cell embryos examined in all experiments. The duration of the fourth cell cycle among these embryos was approximately 26 hours. During development after the 16-cell stage, PDGF reduced the proportion of embryos bastulating from 12.7% to 5.8%; in contrast, transforming growth factor alpha (TGF alpha), acting during the same developmental time period, increased the proportion of embryos blastulating from 8.6% to 40.6%. These results, using serum-free medium, indicated that PDGF signalled completion of the fourth cell cycle. TGF alpha, and perhaps basic fibroblast growth factor (bFGF), promoted blastulation of 16-cell embryos during subsequent culture.

Biologically active precursor for transforming growth factor type alpha, released by retrovirally transformed cells.