Dual transcriptomics of virus-host interactions: comparing two Pacific oyster families presenting contrasted susceptibility to ostreid herpesvirus 1.
Dual transcriptomics of virus-host interactions: comparing two Pacific oyster families presenting contrasted susceptibility to ostreid herpesvirus 1.
复制标题
DOI：
10.1186/1471-2164-15-580
复制
发表时间：
2014-07-09
期刊：
影响因子：
4.4
通讯作者：
Renault T
中科院分区：
文献类型：
--
作者：
Segarra A;Mauduit F;Faury N;Trancart S;Dégremont L;Tourbiez D;Haffner P;Barbosa-Solomieu V;Pépin JF;Travers MA;Renault T
太平洋牡蛎（Crassostrea gigas）在不同国家的大规模死亡暴发与一种名为ostreid herpesvirus type 1（OsHV-1）的疱疹病毒的检测有关。然而，很少有研究已经进行理解和遵循病毒基因表达，因为它已经在脊椎动物疱疹病毒。本研究对C.为了测试几个双亲牡蛎家族对该病毒的易感性，并使用体内转录组学方法分析宿主-病原体相互作用，进行了用OsHV-1感染太平洋牡蛎的试验。这些牡蛎家族在死亡率方面的不同反应证实了对OsHV-1感染的易感性具有显着的遗传成分。选择两个存活率有差异的家系。通过实时荧光定量PCR共检测了39个病毒基因和5个宿主基因。初步结果提供了关于（i）基于病毒DNA复制和转录动力学的OsHV-1的病毒周期和（ii）宿主对病毒的防御机制的信息。两个家系中病毒DNA和RNA的检出量有显著性差异。这一结果表明，太平洋牡蛎在对OsHV-1感染的易感性方面具有遗传多样性。这种对比敏感性与不同的宿主基因表达谱有关。此外，本研究表明，病毒DNA量和所选牡蛎基因的表达水平之间呈正相关。
Massive mortality outbreaks affecting Pacific oyster (Crassostrea gigas) spat in various countries have been associated with the detection of a herpesvirus called ostreid herpesvirus type 1 (OsHV-1). However, few studies have been performed to understand and follow viral gene expression, as it has been done in vertebrate herpesviruses. In this work, experimental infection trials of C. gigas spat with OsHV-1 were conducted in order to test the susceptibility of several bi-parental oyster families to this virus and to analyze host-pathogen interactions using in vivo transcriptomic approaches. The divergent response of these oyster families in terms of mortality confirmed that susceptibility to OsHV-1 infection has a significant genetic component. Two families with contrasted survival rates were selected. A total of 39 viral genes and five host genes were monitored by real-time PCR. Initial results provided information on (i) the virus cycle of OsHV-1 based on the kinetics of viral DNA replication and transcription and (ii) host defense mechanisms against the virus. In the two selected families, the detected amounts of viral DNA and RNA were significantly different. This result suggests that Pacific oysters are genetically diverse in terms of their susceptibility to OsHV-1 infection. This contrasted susceptibility was associated with dissimilar host gene expression profiles. Moreover, the present study showed a positive correlation between viral DNA amounts and the level of expression of selected oyster genes.
登录
查看更多内容
影响因子：
4.4
作者：
Jouaux A;Lafont M;Blin JL;Houssin M;Mathieu M;Lelong C
通讯作者：
Lelong C
影响因子：
3.8
作者：
Chu, JJH;Ng, ML
通讯作者：
Ng, ML
影响因子：
2.7
作者：
Davison, Andrew J.;Eberle, Richard;Ehlers, Bernhard;Hayward, Gary S.;McGeoch, Duncan J.;Minson, Anthony C.;Pellett, Philip E.;Roizman, Bernard;Studdert, Michael J.;Thiry, Etienne
通讯作者：
Thiry, Etienne
影响因子：
3.8
作者：
Davison, AJ;Trus, BL;Renault, T
通讯作者：
Renault, T
影响因子：
4.5
作者：
Degremont, Lionel;Bedier, Edouard;Boudry, Pierre
通讯作者：
Boudry, Pierre