Integrated confocal and scanning probe microscopy for biomedical research.

Integrated confocal and scanning probe microscopy for biomedical research.
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DOI:
10.1100/tsw.2006.269
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发表时间:
2006-12-15
影响因子:
--
通讯作者:
Horton MA
Horton MA
中科院分区:
其他
文献类型:
--
作者:
Haupt BJ;Pelling AE;Horton MA

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原子力显微镜(AFM)不仅在设计上,而且在应用上都在不断发展。利用原子力显微镜的新重点正在从纯材料转向生物医学研究。更常见的是,它与其他光学成像方法结合使用,如共聚焦激光扫描显微镜(CLSM)和荧光成像,以提供对生物系统更全面的了解。迄今为止,AFM已越来越多地被用作精确的显微操作器,探测和改变活细胞和组织的机械生物学特性,以检查特异性,受体-配体相互作用,材料特性和细胞行为。本文就这种新型杂交原子力显微镜的发展、研究现状及其在疾病诊断和检测方面的潜在应用作一综述。
Atomic force microscopy (AFM) continues to be developed, not only in design, but also in application. The new focus of using AFM is changing from pure material to biomedical studies. More frequently, it is being used in combination with other optical imaging methods, such as confocal laser scanning microscopy (CLSM) and fluorescent imaging, to provide a more comprehensive understanding of biological systems. To date, AFM has been used increasingly as a precise micromanipulator, probing and altering the mechanobiological characteristics of living cells and tissues, in order to examine specific, receptor-ligand interactions, material properties, and cell behavior. In this review, we discuss the development of this new hybrid AFM, current research, and potential applications in diagnosis and the detection of disease.
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