Single-cell elastography: probing for disease with the atomic force microscope.
Single-cell elastography: probing for disease with the atomic force microscope.
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DOI：
10.1155/2004/482680
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Costa KD
中科院分区：
文献类型：
--
作者：
Costa KD
原子力显微镜（AFM）是细胞生物学研究中的一个重要工具。AFM最初是为了高分辨率成像而开发的，它还具有独特的纳米压痕能力，可以探测培养中活细胞的动态粘弹性材料特性。特别是，AFM弹性成像结合了成像和压痕模式，以映射细胞力学特性的空间分布，这反过来又反映了底层细胞骨架的结构和功能。这种测量有助于我们理解细胞力学和细胞生物学，并且似乎对单个细胞中疾病的存在很敏感。本章提供了AFM弹性成像的原理和实践的背景，并回顾了文献比较细胞力学在正常和患病状态，使一个案件使用这种测量作为疾病标志物。重点是需要更全面和详细的量化细胞生物力学性能超出目前的标准分析方法。在该方法可以用于临床之前，还需要克服许多技术和实践障碍。然而，未来有很大的希望AFM弹性成像的活细胞提供新的生物力学标记，将加强检测，诊断和治疗疾病。
The atomic force microscope (AFM) is emerging as a powerful tool in cell biology. Originally developed for high-resolution imaging purposes, the AFM also has unique capabilities as a nano-indenter to probe the dynamic viscoelastic material properties of living cells in culture. In particular, AFM elastography combines imaging and indentation modalities to map the spatial distribution of cell mechanical properties, which in turn reflect the structure and function of the underlying cytoskeleton. Such measurements have contributed to our understanding of cell mechanics and cell biology and appear to be sensitive to the presence of disease in individual cells. This chapter provides a background on the principles and practice of AFM elastography and reviews the literature comparing cell mechanics in normal and diseased states, making a case for the use of such measurements as disease markers. Emphasis is placed on the need for more comprehensive and detailed quantification of cell biomechanical properties beyond the current standard methods of analysis. A number of technical and practical hurdles have yet to be overcome before the method can be of clinical use. However, the future holds great promise for AFM elastography of living cells to provide novel biomechanical markers that will enhance the detection, diagnosis, and treatment of disease.
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