Detection of group 1 coronaviruses in bats in North America.

Detection of group 1 coronaviruses in bats in North America.
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DOI:
10.3201/eid1309.070491
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发表时间:
2007-09
影响因子:
11.8
通讯作者:
Holmes KV
Holmes KV
中科院分区:
医学2区
文献类型:
--
作者:
Dominguez SR;O'Shea TJ;Oko LM;Holmes KV

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2种蝙蝠携带1组冠状病毒。严重急性呼吸综合征(SARS)是由一种新型冠状病毒(SARS-CoV)引起的。中华人民共和国南部的几种蝙蝠携带SARS样冠状病毒,可能是它们的储存宿主。为了确定北美的蝙蝠是否也携带冠状病毒,我们使用逆转录-PCR检测蝙蝠中的冠状病毒RNA。我们在28份粪便标本中的6份中发现了冠状病毒RNA,这些粪便标本来自测试的7种蝙蝠中的2种。病毒RNA脱落的流行率很高:在棕蝠中为17%,在鼠耳蝠中为50%。基因1b中440 bp扩增子的序列分析表明,这些落基山蝙蝠冠状病毒在系统发育组1中形成3个簇,与亚洲蝙蝠冠状病毒组1不同。由于蝙蝠冠状病毒有可能在人类和动物中引起疾病,因此需要在北美对蝙蝠冠状病毒进行进一步监测和表征。
Bats of 2 species harbor group 1 coronaviruses. The epidemic of severe acute respiratory syndrome (SARS) was caused by a newly emerged coronavirus (SARS-CoV). Bats of several species in southern People’s Republic of China harbor SARS-like CoVs and may be reservoir hosts for them. To determine whether bats in North America also harbor coronaviruses, we used reverse transcription–PCR to detect coronavirus RNA in bats. We found coronavirus RNA in 6 of 28 fecal specimens from bats of 2 of 7 species tested. The prevalence of viral RNA shedding was high: 17% in Eptesicus fuscus and 50% in Myotis occultus. Sequence analysis of a 440-bp amplicon in gene 1b showed that these Rocky Mountain bat coronaviruses formed 3 clusters in phylogenetic group 1 that were distinct from group 1 coronaviruses of Asian bats. Because of the potential for bat coronaviruses to cause disease in humans and animals, further surveillance and characterization of bat coronaviruses in North America are needed.
DOI: 10.3201/eid0101.950107
发表时间: 1995-01
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