The Histone H1-Like Protein AlgP Facilitates Even Spacing of Polyphosphate Granules in Pseudomonas aeruginosa.
The Histone H1-Like Protein AlgP Facilitates Even Spacing of Polyphosphate Granules in Pseudomonas aeruginosa.
复制标题
作者：
多聚磷酸盐的合成是细菌对胁迫和饥饿的一种古老而普遍的反应。在许多细菌中，polyP链聚集在一起形成细胞内的颗粒状超结构。一些物种似乎调节聚P颗粒亚细胞组织。尽管polyP在饥饿适应性中起着关键作用，但这些结构的组成、支撑其组织的机制以及这种组织的功能意义仍知之甚少。我们以前确定，颗粒成为短暂的均匀分布在细胞的长轴在氮饥饿的机会人类病原体铜绿假单胞菌。在这里，我们开发了一种颗粒富集方案来筛选聚P颗粒定位蛋白。我们将AlgP鉴定为与polyP颗粒相关的蛋白质。我们进一步发现，AlgP是聚P颗粒均匀间隔所必需的。AlgP是一种DNA结合蛋白，具有154个氨基酸的C-末端结构域，富含“KPAA”重复序列和该重复序列的变体，其总体序列组成类似于真核组蛋白H1的C-末端尾部。颗粒的大小，数量和间距显着扰动的AlgP的情况下，或当AlgP被截断删除的C-末端。ΔalgP和algPΔCTD突变体具有更少、更大的颗粒。我们推测，AlgP可能有助于间距拴聚P颗粒的染色体，从而抑制与相邻颗粒的融合。我们的发现，AlgP促进颗粒间距允许我们第一次直接解耦颗粒生物起源均匀间距，并将告知未来的努力，探索功能的意义，颗粒组织在饥饿期间健身。
Synthesis of polyphosphate (polyP) is an ancient and universal stress and starvation response in bacteria. In many bacteria, polyP chains come together to form granular superstructures within cells. Some species appear to regulate polyP granule subcellular organization. Despite the critical role of polyP in starvation fitness, the composition of these structures, mechanism(s) underpinning their organization, and functional significance of such organization are poorly understood. We previously determined that granules become transiently evenly spaced on the cell’s long axis during nitrogen starvation in the opportunistic human pathogen Pseudomonas aeruginosa. Here, we developed a granule-enrichment protocol to screen for polyP granule-localizing proteins. We identified AlgP as a protein that associates with polyP granules. We further discovered that AlgP is required for the even spacing of polyP granules. AlgP is a DNA-binding protein with a 154 amino acid C-terminal domain enriched in “KPAA” repeats and variants of this repeat, with an overall sequence composition similar to the C-terminal tail of eukaryotic histone H1. Granule size, number, and spacing are significantly perturbed in the absence of AlgP, or when AlgP is truncated to remove the C-terminus. The ΔalgP and algPΔCTD mutants have fewer, larger granules. We speculate that AlgP may contribute to spacing by tethering polyP granules to the chromosome, thereby inhibiting fusion with neighboring granules. Our discovery that AlgP facilitates granule spacing allows us for the first time to directly uncouple granule biogenesis from even spacing, and will inform future efforts to explore the functional significance of granule organization on fitness during starvation.
登录
查看更多内容
DOI：
10.1139/bcb-2016-0097
发表时间：
2017-04
期刊：
Biochemistry and cell biology = Biochimie et biologie cellulaire
影响因子：
--
作者：
Cutter AR;Hayes JJ
通讯作者：
Hayes JJ
影响因子：
64.8
作者：
Jumper J;Evans R;Pritzel A;Green T;Figurnov M;Ronneberger O;Tunyasuvunakool K;Bates R;Žídek A;Potapenko A;Bridgland A;Meyer C;Kohl SAA;Ballard AJ;Cowie A;Romera-Paredes B;Nikolov S;Jain R;Adler J;Back T;Petersen S;Reiman D;Clancy E;Zielinski M;Steinegger M;Pacholska M;Berghammer T;Bodenstein S;Silver D;Vinyals O;Senior AW;Kavukcuoglu K;Kohli P;Hassabis D
通讯作者：
Hassabis D
影响因子：
3.4
作者：
Azaldegui, Christopher A.;Vecchiarelli, Anthony G.;Biteen, Julie S.
通讯作者：
Biteen, Julie S.
影响因子：
3.2
作者：
Butan, Carmen;Hartnell, Lisa M.;Milne, Jacqueline L. S.
通讯作者：
Milne, Jacqueline L. S.
影响因子：
3.2
作者：
DERETIC, V;KONYECSNI, WM
通讯作者：
KONYECSNI, WM