Epitope-associated and specificity-focused features of EV71-neutralizing antibody repertoires from plasmablasts of infected children.
Epitope-associated and specificity-focused features of EV71-neutralizing antibody repertoires from plasmablasts of infected children.
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DOI：
10.1038/s41467-017-00736-9
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发表时间：
2017-10-02
影响因子：
16.6
通讯作者：
Lin TY
中科院分区：
文献类型：
--
作者：
Arthur Huang KY;Chen MF;Huang YC;Shih SR;Chiu CH;Lin JJ;Wang JR;Tsao KC;Lin TY
保护性抗体水平对于保护免受严重肠道病毒71感染至关重要。然而，对肠道病毒71型特异性抗体的特异性和功能特性知之甚少。在这里，我们的特点191浆母细胞衍生的单克隆抗体从三个肠道病毒71感染的儿童，每个人都表现出不同的血清学反应。在84种肠道病毒71型特异性抗体中，靶向衣壳峡谷边缘和底部的中和抗体对1998-2016年分离的病毒表现出纳克水平的广泛和有效活性。我们还发现一个子集的感染儿童的肠道病毒71特异性抗体集中在3-和2-倍的平台表位定位在边缘的五聚体，这种类型的抗体反应与较低的血清滴度对最近流行的菌株。我们的数据提供了新的见解肠道病毒71型特异性抗体诱导的自然感染在血清学和克隆水平。肠道病毒71是手足口病和疱疹性咽峡炎的主要病因。在这里，作者表征了一大组浆母细胞衍生的IgG mAb，其靶向EV 71的衣壳以鉴定由自然感染诱导的中和抗体。
Protective antibody levels are critical for protection from severe enterovirus 71 infection. However, little is known about the specificities and functional properties of the enterovirus 71-specific antibodies induced by natural infection in humans. Here we characterize 191 plasmablast-derived monoclonal antibodies from three enterovirus 71-infected children, each of whom shows a distinct serological response. Of the 84 enterovirus 71-specific antibodies, neutralizing antibodies that target the rims and floor of the capsid canyon exhibit broad and potent activities at the nanogram level against viruses isolated in 1998–2016. We also find a subset of infected children whose enterovirus 71-specific antibodies are focused on the 3- and 2-fold plateau epitopes localized at the margin of pentamers, and this type of antibody response is associated with lower serum titers against recently circulating strains. Our data provide new insights into the enterovirus 71-specific antibodies induced by natural infection at the serological and clonal levels. Enterovirus 71 is a leading cause of hand-foot-and-mouth disease and herpangina. Here, the authors characterize a large panel of plasmablast-derived IgG mAbs that target the capsid of EV71 to identify neutralizing antibodies induced by natural infection.
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DOI：
10.1084/jem.20090766
发表时间：
2010-08-30
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Fafi-Kremer S;Fofana I;Soulier E;Carolla P;Meuleman P;Leroux-Roels G;Patel AH;Cosset FL;Pessaux P;Doffoël M;Wolf P;Stoll-Keller F;Baumert TF
通讯作者：
Baumert TF
影响因子：
11.1
作者：
Fibriansah, Guntur;Tan, Joanne L.;Smith, Scott A.;de Alwis, Adamberage R.;Thiam-Seng Ng;Kostyuchenko, Victor A.;Ibarra, Kristie D.;Wang, Jiaqi;Harris, Eva;de Silva, Aravinda;Crowe, James E., Jr.;Lok, Shee-Mei
通讯作者：
Lok, Shee-Mei
影响因子：
30.3
作者：
Jackson KJ;Liu Y;Roskin KM;Glanville J;Hoh RA;Seo K;Marshall EL;Gurley TC;Moody MA;Haynes BF;Walter EB;Liao HX;Albrecht RA;García-Sastre A;Chaparro-Riggers J;Rajpal A;Pons J;Simen BB;Hanczaruk B;Dekker CL;Laserson J;Koller D;Davis MM;Fire AZ;Boyd SD
通讯作者：
Boyd SD
影响因子：
19
作者：
Fischer, T. K.;Nielsen, A. Y.;Midgley, S. E.
通讯作者：
Midgley, S. E.
影响因子：
5.4
作者：
Brown, BA;Oberste, MS;Pallansch, MA
通讯作者：
Pallansch, MA