Shotgun sequencing of the vaginal microbiome reveals both a species and functional potential signature of preterm birth.
Shotgun sequencing of the vaginal microbiome reveals both a species and functional potential signature of preterm birth.
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DOI：
10.1038/s41522-020-00162-8
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发表时间：
2020-11-12
影响因子：
9.2
通讯作者：
Cotter PD
中科院分区：
文献类型：
--
作者：
Feehily C;Crosby D;Walsh CJ;Lawton EM;Higgins S;McAuliffe FM;Cotter PD
几项研究报告了阴道微生物区系与早产(PTB)的关系。从乳酸菌的高比例到混合物种群落的种群转移，就像细菌性阴道病一样，与肺结核的风险增加了两倍有关。尽管越来越多的研究使用下一代测序技术，主要是基于16S rRNA的方法来研究怀孕期间的阴道微生物区系，但没有明显的微生物特征与肺结核有关。散弹枪元基因组测序为揭示群落结构及其基因功能提供了一个强大的工具，其分辨率远远高于扩增子测序。在这项研究中，我们使用鸟枪元基因组测序来比较早产高危妇女(n = 35)和低风险对照组(n = 14)的阴道微生物区系。虽然微生物多样性和丰富度在不同组之间没有差异，但在个体物种方面存在显著差异。特别是，皱纹乳杆菌与足月妊娠的样本有关，而一种社区状态类型与早产的样本有关。此外，通过预测基因功能，早产微生物区系的功能潜力不同于完全等值的微生物区系。综上所述，我们观察到早产妇女阴道微生物组成在结构和功能上的离散差异。重要：据估计，每年有1500万例自发性早产，是五岁以下婴儿死亡的主要原因。因此，准确识别有自发性肺结核风险的妊娠是至关重要的。然而，没有单一的原因可归因于。微生物感染是一个已知的危险因素，但对阴道微生物的作用知之甚少。使用高分辨率DNA测序技术，我们调查了被认为是肺结核高危女性阴道中存在的微生物群落。我们确认，皱纹乳杆菌与足月妊娠密切相关，而其他微生物群落与肺结核有关。重要的是，我们展示了结核分枝杆菌样本中存在的微生物的特定功能不同于FTB样本，突出了我们测序方法的力量。这些信息使我们能够开始了解可能影响肺结核的特定微生物特征，而不仅仅是微生物分类群的存在或不存在。
An association between the vaginal microbiota and preterm birth (PTB) has been reported in several research studies. Population shifts from high proportions of lactobacilli to mixed species communities, as seen with bacterial vaginosis, have been linked to a twofold increased risk of PTB. Despite the increasing number of studies using next-generation sequencing technologies, primarily involving 16S rRNA-based approaches, to investigate the vaginal microbiota during pregnancy, no distinct microbial signature has been associated with PTB. Shotgun metagenomic sequencing offers a powerful tool to reveal community structures and their gene functions at a far greater resolution than amplicon sequencing. In this study, we employ shotgun metagenomic sequencing to compare the vaginal microbiota of women at high risk of preterm birth (n = 35) vs. a low-risk control group (n = 14). Although microbial diversity and richness did not differ between groups, there were significant differences in terms of individual species. In particular, Lactobacillus crispatus was associated with samples from a full-term pregnancy, whereas one community state-type was associated with samples from preterm pregnancies. Furthermore, by predicting gene functions, the functional potential of the preterm microbiota was different from that of full-term equivalent. Taken together, we observed a discrete structural and functional difference in the microbial composition of the vagina in women who deliver preterm. Importance: with an estimated 15 million cases annually, spontaneous preterm birth (PTB) is the leading cause of death in infants under the age of five years. The ability to accurately identify pregnancies at risk of spontaneous PTB is therefore of utmost importance. However, no single cause is attributable. Microbial infection is a known risk factor, yet the role of vaginal microbes is poorly understood. Using high-resolution DNA-sequencing techniques, we investigate the microbial communities present in the vaginal tracts of women deemed high risk for PTB. We confirm that Lactobacillus crispatus is strongly linked to full-term pregnancies, whereas other microbial communities associate with PTB. Importantly, we show that the specific functions of the microbes present in PTB samples differs from FTB samples, highlighting the power of our sequencing approach. This information enables us to begin understanding the specific microbial traits that may be influencing PTB, beyond the presence or absence of microbial taxa.
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影响因子：
5.8
作者：
Gu, Zuguang;Eils, Roland;Schlesner, Matthias
通讯作者：
Schlesner, Matthias
影响因子：
3.7
作者：
Albert, Arianne Y. K.;Chaban, Bonnie;Money, Deborah
通讯作者：
Money, Deborah
早产的妇女具有独特的宫颈阴道代谢组。
影响因子：
9.8
作者：
Ghartey J;Bastek JA;Brown AG;Anglim L;Elovitz MA
通讯作者：
Elovitz MA
DOI：
10.1073/pnas.1502875112
发表时间：
2015-09-01
影响因子：
11.1
作者：
DiGiulio, Daniel B.;Callahan, Benjamin J.;Relman, David A.
通讯作者：
Relman, David A.
影响因子：
7
作者：
Goltsman DSA;Sun CL;Proctor DM;DiGiulio DB;Robaczewska A;Thomas BC;Shaw GM;Stevenson DK;Holmes SP;Banfield JF;Relman DA
通讯作者：
Relman DA