An in vitro biofilm model to examine the effect of antibiotic ointments on biofilms produced by burn wound bacterial isolates.
An in vitro biofilm model to examine the effect of antibiotic ointments on biofilms produced by burn wound bacterial isolates.
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DOI：
10.1016/j.burns.2010.09.017
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发表时间：
2011-03
期刊：
影响因子：
--
通讯作者：
Hamood AN
中科院分区：
文献类型：
--
作者：
Hammond AA;Miller KG;Kruczek CJ;Dertien J;Colmer-Hamood JA;Griswold JA;Horswill AR;Hamood AN
烧伤创面的局部治疗是必不可少的，因为烧伤组织中的血液供应减少限制了全身抗生素的作用。在烧伤表面上，微生物存在于称为生物膜的复杂结构中，其显著增强细菌对抗菌剂的抗性。由于细菌形成生物膜的能力不同，这些生物膜对局部应用的抗生素的敏感性也不同，因此必须确定哪些局部抗生素能有效地破坏或防止这些病原体产生的生物膜。然而，一个简单的体外试验，比较烧伤创面分离株产生的生物膜不同的局部抗生素的敏感性还没有报道。通过将纤维素盘与烧伤伤口分离物一起接种在琼脂平板上并孵育24小时来形成生物膜。然后将生物膜用未处理的纱布或涂有抗生素软膏的纱布覆盖24小时，并对剩余的微生物进行定量并在显微镜下观察。莫匹罗星和三联抗生素软膏显著减少了由测试的金黄色葡萄球菌和铜绿假单胞菌烧伤伤口分离株产生的生物膜，与庆大霉素软膏一样，除了一种铜绿假单胞菌临床分离株。所描述的测定是鉴定在消除由烧伤伤口分离物产生的生物膜方面最有效的局部抗生素的实用且可再现的方法。
Topical treatment of burn wounds is essential as reduced blood supply in the burned tissues restricts the effect of systemic antibiotics. On the burn surface, microorganisms exist within a complex structure termed a biofilm, which enhances bacterial resistance to antimicrobial agents significantly. Since bacteria differ in their ability to develop biofilms, the susceptibility of these biofilms to topically-applied antibiotics varies, making it essential to identify which topical antibiotics efficiently disrupt or prevent biofilms produced by these pathogens. Yet, a simple in vitro assay to compare the susceptibility of biofilms produced by burn wound isolates to different topical antibiotics has not been reported. Biofilms were developed by inoculating cellulose disks on agar plates with burn wound isolates and incubating for 24 h. The biofilms were then covered for 24 h with untreated gauze or gauze coated with antibiotic ointment and remaining microorganisms were quantified and visualized microscopically. Mupirocin and triple antibiotic ointments significantly reduced biofilms produced by the Staphylococcus aureus and Pseudomonas aeruginosa burn wound isolates tested, as did gentamicin ointment, with the exception of one P. aeruginosa clinical isolate. The described assay is a practical and reproducible approach to identify topical antibiotics most effective in eliminating biofilms produced by burn wound isolates.
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影响因子：
3.6
作者：
Barret, JP;Herndon, DN
通讯作者：
Herndon, DN
影响因子：
9.4
作者：
Hogardt, M;Trebesius, K;Heesemann, J
通讯作者：
Heesemann, J
DOI：
10.3109/02844318709086457
发表时间：
1987-01-01
影响因子：
--
作者：
MANSON, WL;WESTERVELD, AW;SAUER, EW
通讯作者：
SAUER, EW
影响因子：
2.1
作者：
Freestone, PPE;Haigh, RD;Lyte, M
通讯作者：
Lyte, M
影响因子：
2.7
作者：
Kennedy, Peter;Brammah, Susan;Wills, Edward
通讯作者：
Wills, Edward