Application of a dye-based mitochondrion-thermometry to determine the receptor downstream of prostaglandin E(2) involved in the regulation of hepatocyte metabolism.
Application of a dye-based mitochondrion-thermometry to determine the receptor downstream of prostaglandin E(2) involved in the regulation of hepatocyte metabolism.
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应用基于染料的线粒体测温法测定参与肝细胞代谢调节的前列腺素 E-2 下游受体
DOI：
10.1038/s41598-018-31356-y
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发表时间：
2018-08-30
影响因子：
4.6
通讯作者：
Kang JS
中科院分区：
文献类型：
--
作者：
Shen L;Xie TR;Yang RZ;Chen Y;Kang JS
活细胞内的温度分布反映了细胞组分的热力学和功能。我们使用了一种新开发的基于罗丹明B甲酯的线粒体测温方法，该方法作为非荧光和荧光共振形式的热敏混合物进行平衡。前列腺素E2（PGE 2）是从肝非实质枯否细胞释放的，并作为一种炎症因子影响肝细胞的各种功能。PGE 2对肝细胞能量机制的作用尚未完全阐明，特别是PGE 2受体介导的功能一直是难以捉摸的。我们通过我们的电子体温测量方法确定EP 4为PGE 2的主要受体，然后证实该受体在肝脏代谢中的作用。我们发现PGE 2可通过EP 4受体增加某些脂肪生成基因的表达，抑制脂肪分解和线粒体β-氧化，降低细胞内ATP水平，升高cAMP水平，从而降低肝细胞内温度。PGE 2以EP 4受体依赖性方式影响肝细胞的氧化还原状态，其表示为FAD与FAD + NADH的比率。总之，这些数据表明PGE 2主要通过EP 4受体调节肝细胞的代谢。
Temperature distributions inside a living cell reflect the thermodynamics and functions of cellular components. We used a newly-developed method of mitochondrial thermometry based on Rhodamine B methyl ester, which equilibrates as a thermosensitive mixture of nonfluorescent and fluorescent resonance forms. Prostaglandin E2 (PGE2) is released from hepatic non-parenchymal Kupffer cells and acts as an inflammatory factor to impact various functions of hepatocytes. The activity of PGE2 on energy mechanism of hepatocytes has not been fully elucidated and in particular, which PGE2 receptor mediates the functions has been elusive. We identified EP4 as the major receptor of PGE2 via our mitochondrion-thermometry approach and then substantiated this receptor’s role in hepatic metabolism. We discovered that PGE2 is able to decrease intracellular temperature of hepatocytes, via increasing some lipogenic genes’ expressions, hampering lipolysis and mitochondrial β-oxidation, reducing intracellular ATP level and elevating cAMP level through EP4 receptor. The redox status of hepatocytes represented by FAD vs FAD + NADH ratio is influenced by PGE2 in an EP4 receptor-dependent manner. Collectively, these data demonstrate that PGE2 regulates metabolism of hepatocytes mainly through EP4 receptor.
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影响因子：
4.6
作者：
Arai S;Lee SC;Zhai D;Suzuki M;Chang YT
通讯作者：
Chang YT
影响因子：
48
作者：
Baffou, Guillaume;Rigneault, Herve;Jullien, Ludovic
通讯作者：
Jullien, Ludovic
荧光比率测定和荧光寿命成像：使用单分子传感器进行细胞粘度的双模式成像
影响因子：
15
作者：
Peng, Xiaojun;Yang, Zhigang;Yang, Meng
通讯作者：
Yang, Meng
DOI：
10.1016/j.bbalip.2006.02.003
发表时间：
2006-02-01
影响因子：
4.8
作者：
Pérez, S;Aspichueta, P;Chico, Y
通讯作者：
Chico, Y
DOI：
10.1073/pnas.77.2.990
发表时间：
1980-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
JOHNSON, LV;WALSH, ML;CHEN, LB
通讯作者：
CHEN, LB