Isolation and characterization of microsatellite loci in the invasive herb Solidago altissima (Asteraceae).

Isolation and characterization of microsatellite loci in the invasive herb Solidago altissima (Asteraceae).
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DOI:
10.3732/apps.1200313
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发表时间:
2013-04
影响因子:
3.6
通讯作者:
Isagi Y
Isagi Y
中科院分区:
生物学4区
文献类型:
--
作者:
Sakata Y;Kaneko S;Hayano A;Inoue-Murayama M;Ohgushi T;Isagi Y

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• 研究前提:在日本引入范围内的多年生草本植物一枝黄花中开发了微卫星标记,以评估其种群结构并促进入侵扩张的跟踪。 • 方法和结果:使用454 焦磷酸测序,为S. altissima 开发了16 个微卫星引物组。引物组在日本三个人群中抽取的 70 名个体上进行了测试。引物扩增了每个基因座 5 至 25 个等位基因的二核苷酸和三核苷酸重复序列,预期杂合度范围为 0.46 至 0.92。 • 结论:这些结果表明,S. altissima 中的引物可用于未来广泛应用的研究,包括跟踪入侵动态和研究该物种的群体遗传学。
• Premise of the study: Microsatellite markers were developed in the perennial herb Solidago altissima from populations within its introduced range in Japan to assess its population structure and to facilitate tracking of invasion expansion. • Methods and Results: Using 454 pyrosequencing, 16 microsatellite primer sets were developed for S. altissima. The primer sets were tested on 70 individuals sampled from three populations in Japan. The primers amplified di- and trinucleotide repeats with five to 25 alleles per locus, and the expected heterozygosity ranged from 0.46 to 0.92. • Conclusions: These results indicate the utility of primers in S. altissima for future research on a wide range of applications, including tracking of invasion dynamics and investigating population genetics of the species.
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