Vaults are dynamically unconstrained cytoplasmic nanoparticles capable of half vault exchange.
Vaults are dynamically unconstrained cytoplasmic nanoparticles capable of half vault exchange.
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穹窿是动态不受约束的细胞质纳米颗粒，能够进行半穹窿交换。
DOI：
10.1021/nn102051r
复制
发表时间：
2010
期刊：
影响因子：
17.1
通讯作者：
Rome,LeonardH
中科院分区：
文献类型：
--
作者：
Yang,Jian;Kickhoefer,ValerieA;Ng,BennyC;Gopal,Ajaykumar;Bentolila,LaurentA;John,Scott;Tolbert,SarahH;Rome,LeonardH
拱顶是天然存在的核糖核蛋白颗粒，具有巨大的内部体积，大到足以封装数百种蛋白质。它们是高度保守的，存在于几乎所有的真核细胞中，每个细胞从104到107个颗粒。重组库可以在体外产生，并被设计成允许细胞靶向和蛋白质包装。这些纳米尺寸的颗粒具有许多理想的特性，这些特性可以使它们具有用作药物递送载体的优势。使用光活化绿色荧光蛋白（PAGFP）标记的拱顶，我们证明，在活细胞中的单像素光活化后，颗粒迅速扩散到整个细胞质。尽管暴露于各种细胞应力，它们的体内运动保持相对不变，这表明穹窿在很大程度上不受细胞质的约束。荧光共振能量转移（FRET）从聚乙二醇（PEG）融合的杂交细胞中观察到，表达CFP或YFP标记的穹窿，表明穹窿可以在体内交换主要穹窿蛋白（MVP）亚基。对这种交换机制的研究表明，它们能够在粒子腰部快速分离并重新组装成整个拱顶，支持半拱顶交换机制。这些数据表明了一种方法，使金库可以在功能上与其细胞环境相互作用，并运送包装在其内部的材料。
Vaults are naturally occurring ribonucleoprotein particles with an enormous interior volume, large enough to encapsulate hundreds of proteins. They are highly conserved and are present in nearly all eukaryotic cells ranging from 104to 107particles per cell. Recombinant vaults can be producedin vitroand engineered to allow cell targeting and protein packaging. These nanometer-sized particles have many desirable characteristics that may give them advantages for use as drug delivery vehicles. Using photoactivatable green fluorescent protein (PAGFP) labeled vaults, we demonstrate that the particles rapidly diffuse throughout the cytoplasm following single pixel photoactivation in live cells. Theirin vivomovement remained relatively unchanged despite exposure to a variety of cellular stresses, suggesting that vaults are largely unconstrained in the cytoplasm. Fluorescence resonance energy transfer (FRET) was observed from polyethylene glycol (PEG) fused hybrid cells that expressed either CFP or YFP labeled vaults, indicating that vaults can exchange major vault protein (MVP) subunitsin vivo. Investigation into the mechanism of this exchangein vitrousing recombinant vaults demonstrated that they were capable of rapidly separating at the particle waist and reassembling back into whole vaults, supporting a half vault exchange mechanism. This data suggests a means whereby vaults can functionally interact with their cellular environment and deliver materials packaged within their interior.
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DOI：
10.1083/jcb.103.3.699
发表时间：
1986-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kedersha NL;Rome LH
通讯作者：
Rome LH
影响因子：
17.1
作者：
Goldsmith, Lisa E.;Pupols, Melody;Monbouquette, Harold G.
通讯作者：
Monbouquette, Harold G.
影响因子：
56.9
作者：
Patterson, GH;Lippincott-Schwartz, J
通讯作者：
Lippincott-Schwartz, J
影响因子：
2.9
作者：
Humphrey, W;Dalke, A;Schulten, K
通讯作者：
Schulten, K
拱顶管的形成：拱顶和拱顶 PARP 之间的动态相互作用
影响因子：
4
作者：
A. van Zon;Marieke H. Mossink;M. Schoester;A. Houtsmuller;G. Scheffer;R. Scheper;P. Sonneveld;E. Wiemer
通讯作者：
E. Wiemer