From molecular to macroscopic via the rational design of a self-assembled 3D DNA crystal.
From molecular to macroscopic via the rational design of a self-assembled 3D DNA crystal.
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我们生活在一个宏观的三维世界中，但我们对物质结构的最佳描述是在原子和分子尺度上。理解这两个尺度之间的关系需要我们从分子世界到宏观世界的桥梁。以原子精度连接这两个领域是自然科学的核心目标，但它需要对物质的3D结构进行高度的空间控制。生产精确设计的3D宏观物体的最简单的实际途径是通过自组装形成晶体排列，因为这样的周期性阵列只有概念上简单的要求：[1]其3D结构是鲁棒的基序，[2]当基序自缔合时，基序部分之间的主要亲和力相互作用，以及[3]这些亲和力相互作用的可预测结构。满足所有这些标准来产生3D周期系统并不容易，但它应该很容易通过由粘性末端尾部的结构良好的分支DNA基序来实现。互补的粘性末端优先彼此结合，并在它们这样做时呈现出众所周知的B-DNA结构; DNA的螺旋重复性质有助于构建周期性阵列。关键是与粘性末端相关的传播方向不共享同一平面，而是延伸形成物质的3D排列。在这里，我们报告的晶体结构在4纳米分辨率的设计，自组装，3D晶体的基础上的DNA张拉整体三角形。这些数据清楚地表明，可以通过精确控制来设计和自组装有序的大分子3D晶格。
We live in a macroscopic three-dimensional world, but our best description of the structure of matter is at the atomic and molecular scale. Understanding the relationship between the two scales requires that we bridge from the molecular world to the macroscopic world. Connecting these two domains with atomic precision is a central goal of the natural sciences, but it requires high spatial control of the 3D structure of matter. The simplest practical route to producing precisely designed 3D macroscopic objects is to form a crystalline arrangement by self-assembly, because such a periodic array has only conceptually simple requirements: [1] A motif whose 3D structure is robust, [2] dominant affinity interactions between parts of the motif when it self-associates, and [3] a predictable structures for these affinity interactions. Fulfilling all these criteria to produce a 3D periodic system is not easy, but it should readily be achieved by well-structured branched DNA motifs tailed by sticky ends. Complementary sticky ends associate with each other preferentially and assume the well-known B-DNA structure when they do so; the helically repeating nature of DNA facilitates the construction of a periodic array. It is key that the directions of propagation associated with the sticky ends not share the same plane, but extend to form a 3D arrangement of matter. Here, we report the crystal structure at 4 Å resolution of a designed, self-assembled, 3D crystal based on the DNA tensegrity triangle. The data demonstrate clearly that it is possible to design and self-assemble a well-ordered macromolecular 3D crystalline lattice with precise control.
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影响因子：
38.3
作者：
通讯作者：
--
影响因子：
15
作者：
Liu, D;Wang, MS;Mao, CD
通讯作者：
Mao, CD
影响因子：
15
作者：
Furukawa, Hiroyasu;Kim, Jaheon;Yaghi, Omar M.
通讯作者：
Yaghi, Omar M.
影响因子：
15
作者：
Paukstelis, Paul J.
通讯作者：
Paukstelis, Paul J.
DOI：
10.1073/pnas.97.8.3971
发表时间：
2000-04-11
影响因子：
11.1
作者：
Eichman, BF;Vargason, JM;Ho, PS
通讯作者：
Ho, PS