RNA interference-based gene silencing of phytoene synthase impairs growth, carotenoids, and plastid phenotype in Oncidium hybrid orchid.
RNA interference-based gene silencing of phytoene synthase impairs growth, carotenoids, and plastid phenotype in Oncidium hybrid orchid.
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DOI：
10.1186/2193-1801-3-478
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Yeh KW
中科院分区：
文献类型：
--
作者：
Liu JX;Chiou CY;Shen CH;Chen PJ;Liu YC;Jian CD;Shen XL;Shen FQ;Yeh KW
八氢番茄红素合成酶（PSY）是类胡萝卜素生物合成途径中的第一个限速调节酶。为了通过降低类胡萝卜素含量来改变花色模式，将八氢番茄红素脱氢酶-RNAi构建体导入文心兰类原球茎（PLB）。PSY和香叶基合酶基因在转基因兰花中表达下调。表现半矮秆表型，叶色鲜艳绿色。显微解剖显示发育停滞的质体与罕见的基粒。总类胡萝卜素含量降低，光合电子传递效率下降。叶绿素水平和叶绿素生物合成基因的表达，如OgGLUTR和OgCS显着降低。HPLC分析表明，矮秆转化体中赤霉酸和脱落酸的内源水平比野生型植物低4倍。此外，转基因文心兰植物的耐冷性也降低。结果表明，PSY基因表达下调可导致类胡萝卜素、赤霉酸、脱落酸和叶绿素等代谢途径相关酶基因表达的改变，并导致植物生长发育障碍。
Phytoene synthase (PSY) is the first rate-limiting regulatory enzyme in the carotenoid biosynthesis pathway. In order to modify the floral color pattern by reducing carotenoid contents, a phytoene synthase-RNAi construct was delivered into protocorm-like body (PLB) of Oncidium hybrid orchid. The transgenic orchids show down-regulated level of PSY and geranyl synthase gene. They displayed semi-dwarf phenotype and brilliant green leaves. The microscopic anatomy revealed development-arrested plastids with rare grana. The total carotenoid content was decreased and the efficiency of the photosynthetic electron transport was declined. The chlorophyll level and the expression of chlorophyll biosynthetic genes, such as OgGLUTR and OgCS were dramatically reduced. HPLC analysis showed that the endogenous level of gibberellic acid and abscisic acid in the dwarf transformants are 4-fold lower than in wild type plants. In addition, chilling tolerance of the transgenic Oncidium plants was reduced. The data showed that down-regulation of PSY resulted in alterations of gene expression in enzymes involved in many metabolic pathways, such as carotenoid, gibberellic acid, abscisic acid and chlorophyll biosynthetic pathway as well as causes predominant defects in plant growth and development.
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影响因子：
7.4
作者：
Diretto, Gianfranco;Al-Babili, Salim;Giuliano, Giovanni
通讯作者：
Giuliano, Giovanni
影响因子：
7.4
作者：
Li, Faqiang;Vallabhaneni, Ratnakar;Wurtzel, Eleanore T.
通讯作者：
Wurtzel, Eleanore T.
影响因子：
7.4
作者：
Lindgren, LO;Stålberg, KG;Höglund, AS
通讯作者：
Höglund, AS
影响因子：
5.3
作者：
Brandi F;Bar E;Mourgues F;Horváth G;Turcsi E;Giuliano G;Liverani A;Tartarini S;Lewinsohn E;Rosati C
通讯作者：
Rosati C
影响因子：
4
作者：
Chen, Shun-Ying;Chien, Ching-Te;Baskin, Carol C.
通讯作者：
Baskin, Carol C.