Rhythmic cilia changes support SCN neuron coherence in circadian clock
Rhythmic cilia changes support SCN neuron coherence in circadian clock
复制标题
有节律的纤毛变化支持昼夜节律时钟中的 SCN 神经元一致性
作者：
Hai;Sen Li;Yu;Yu;Pei;Li;Guang;Xiao;Min Wu;Zeng;Tingting Li;Huaibin Hu;Jin;Xiao;Jia;Qiu;Kai Wang;Tao Zhang;Tao Zhou;Ai;Xue;Hui
视交叉上核（SCN）通过细胞间耦合驱动生物钟一致性，从而抵抗环境扰动。我们报道初级纤毛是小鼠SCN神经元之间细胞间偶联以维持内部时钟稳健性所必需的。神经质s生成（NMS）神经元的纤毛在丰度和长度上表现出明显的昼夜节律性。在时差条件下，NMS神经元中纤毛发生的基因消融使内部时钟快速相移。缺乏纤毛的SCN切片中单个神经元的昼夜节律在外部扰动后失去了一致性。有节奏的纤毛变化驱动Sonic Hedgehog （Shh）信号和时钟基因表达的振荡。NMS神经元中Shh信号的失活反映了纤毛消融的影响。因此，SCN中的纤毛- shh信号有助于细胞间偶联。生理与日常节律的协调是由视交叉上核（SCN）的神经元调节的，视交叉上核是生物钟的中心起搏器。Tu等人描述了这些神经元纤毛上的一种信号机制，该机制使SCN的单个细胞保持同步（参见Kim和Blackshaw的观点）。SCN神经元初级纤毛的长度和丰度随日光照-暗循环而振荡。纤毛通过形态因子Sonic Hedgehog （SHH）组织信号，而该基因的表达调控是SCN细胞同步的必要条件。在暴露于改变的光周期以诱导实验性时差反应的小鼠中，破坏SHH信号使动物能够更快地适应改变的环境。- l。纤毛上的信号帮助大脑主生物钟中的神经元配对。
The suprachiasmatic nucleus (SCN) drives circadian clock coherence through intercellular coupling, which is resistant to environmental perturbations. We report that primary cilia are required for intercellular coupling among SCN neurons to maintain the robustness of the internal clock in mice. Cilia in neuromedin S–producing (NMS) neurons exhibit pronounced circadian rhythmicity in abundance and length. Genetic ablation of ciliogenesis in NMS neurons enabled a rapid phase shift of the internal clock under jet-lag conditions. The circadian rhythms of individual neurons in cilia-deficient SCN slices lost their coherence after external perturbations. Rhythmic cilia changes drive oscillations of Sonic Hedgehog (Shh) signaling and clock gene expression. Inactivation of Shh signaling in NMS neurons phenocopied the effects of cilia ablation. Thus, cilia-Shh signaling in the SCN aids intercellular coupling. Description Editor’s summary Coordination of physiology with daily rhythms is regulated by the neurons of the suprachiasmatic nucleus (SCN), the central pacemaker of the biological clock. Tu et al. describe a signaling mechanism at cilia in these neurons that keeps the individual cells of the SCN synchronized (see the Perspective by Kim and Blackshaw). The length and abundance of primary cilia in SCN neurons oscillated with daily light-dark cycles. Cilia organize signaling by the morphogen Sonic Hedgehog (SHH), and regulation of the expression of this gene was required for synchrony of the SCN cells. In mice exposed to an altered light cycle to induce experimental jet lag, disrupting SHH signaling allowed the animals to adjust more quickly to the altered environment. —L. Bryan Ray Signaling at cilia helps couple neurons in the master biological clock in the brain.
影响因子：
13.9
作者：
Mohawk JA;Green CB;Takahashi JS
通讯作者：
Takahashi JS
影响因子：
3.3
作者：
Follit, John A.;Tuft, Richard A.;Pazour, Gregory J.
通讯作者：
Pazour, Gregory J.