An Assessment of the Spatial and Temporal Variability of Biological Responses to Municipal Wastewater Effluent in Rainbow Darter (Etheostoma caeruleum) Collected along an Urban Gradient.
An Assessment of the Spatial and Temporal Variability of Biological Responses to Municipal Wastewater Effluent in Rainbow Darter (Etheostoma caeruleum) Collected along an Urban Gradient.
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DOI：
10.1371/journal.pone.0164879
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Servos MR
中科院分区：
文献类型：
--
作者：
Fuzzen ML;Bragg LM;Tetreault GR;Bahamonde PA;Tanna RN;Bennett CJ;McMaster ME;Servos MR
城市污水及其成分，如新出现的令人关注的化学品，通过破坏水生生物的关键内分泌功能，对鱼类种群的可持续性构成潜在威胁。虽然研究表明，水生生物暴露于新出现的化学品群时，生物标记物发生了变化，但这些标记物随时间的变化在野生鱼类中还没有得到充分的描述。本研究的目的是评估生物标志物对MWWE暴露的时空变异性，并测试这些反应在季节和年份之间的一致性。在5年的时间里，我们在加拿大安大略省格兰德河的春季和秋季采集了彩虹鲈（Etheostoma caeruleum）。除了地表水化学（营养物质和选定的药物）外，还采取了跨水平生物组织的措施。激素分泌、性腺发育和双性严重程度的测量结果在时间上是一致的，这表明在MWWE排放物下游收集的雄性鱼的生殖受损。相比之下，女性卵巢发育和激素产生似乎更受城市化的影响，而不是MWWE。基因表达和体细胞指标分别在不同地点和年份之间变化很大，并且就MWWE的总体影响而言不确定。强大的生物监测程序必须在设计和解释结果时考虑这些因素，特别是当生物端点的空间和时间采样有限时。如果考虑到所测量端点的自然变异性，评估污染物和其他压力源对流域鱼类的影响将大大加强。
Municipal wastewater effluent (MWWE) and its constituents, such as chemicals of emerging concern, pose a potential threat to the sustainability of fish populations by disrupting key endocrine functions in aquatic organisms. While studies have demonstrated changes in biological markers of exposure of aquatic organisms to groups of chemicals of emerging concern, the variability of these markers over time has not been sufficiently described in wild fish species. The aim of this study was to assess the spatial and temporal variability of biological markers in response to MWWE exposure and to test the consistency of these responses between seasons and among years. Rainbow darter (Etheostoma caeruleum) were collected in spring and fall seasons over a 5-year period in the Grand River, Ontario, Canada. In addition to surface water chemistry (nutrients and selected pharmaceuticals), measures were taken across levels of biological organization in rainbow darter. The measurements of hormone production, gonad development, and intersex severity were temporally consistent and suggested impaired reproduction in male fish collected downstream of MWWE outfalls. In contrast, ovarian development and hormone production in females appeared to be influenced more by urbanization than MWWE. Measures of gene expression and somatic indices were highly variable between sites and years, respectively, and were inconclusive in terms of the impacts of MWWE overall. Robust biomonitoring programs must consider these factors in both the design and interpretation of results, especially when spatial and temporal sampling of biological endpoints is limited. Assessing the effects of contaminants and other stressors on fish in watersheds would be greatly enhanced by an approach that considers natural variability in the endpoints being measured.
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影响因子：
10.4
作者：
Filby AL;Neuparth T;Thorpe KL;Owen R;Galloway TS;Tyler CR
通讯作者：
Tyler CR
影响因子：
4.5
作者：
Bahamonde, P. A.;Tetreault, G. R.;Munkittrick, K. R.
通讯作者：
Munkittrick, K. R.
影响因子：
3.8
作者：
Blair, RM;Fang, H;Sheehan, DM
通讯作者：
Sheehan, DM
影响因子：
2
作者：
Galloway, B. J.;Munkittrick, K. R.
通讯作者：
Munkittrick, K. R.
影响因子：
2.7
作者：
Bjerregaard, Lisette B.;Madsen, Allan H.;Bjerregaard, Poul
通讯作者：
Bjerregaard, Poul