Sputter Deposition of Titanium on Poly(Methyl Methacrylate) Enhances Corneal Biocompatibility.
Sputter Deposition of Titanium on Poly(Methyl Methacrylate) Enhances Corneal Biocompatibility.
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DOI：
10.1167/tvst.9.13.41
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发表时间：
2020-12
影响因子：
3
通讯作者：
Chodosh J
中科院分区：
文献类型：
--
作者：
Sharifi S;Islam MM;Sharifi H;Islam R;Nilsson PH;Dohlman CH;Mollnes TE;Paschalis EI;Chodosh J
评价Boston人工角膜（BK）的聚甲基丙烯酸甲酯（PMMA）柄的钛（Ti）溅射作为增强PMMA与受体角膜组织之间界面粘附的方法。用Ar/O2等离子体处理PMMA试样，并使用DC磁控溅射仪涂覆Ti。分别用原子力显微镜和水接触角仪对表面形貌和亲水性进行了表征。使用机械测试仪测量Ti膜的划痕硬度和附着力。使用体外培养的人角膜成纤维细胞和全血进行生物相容性评估。使用定制的光具座评价Ti溅射的BK的光学质量。通过接触角研究，钛涂层改善了PMMA的亲水性，以匹配医用级钛（Ti-6Al-4V-ELI）。接触表面的Ti溅射导致具有增加的表面粗糙度的板状形态，而不影响BK光学部件的透明度。划痕试验表明，钛涂层的机械性能与铸造钛相似，涂层在拉脱附着力试验中稳定。基于细胞活力、粘附、增殖、分化、胶原沉积和角膜蛋白聚糖表达的测试，溅射的钛膜具有高度生物相容性，其性质超过未涂覆的PMMA，并且不会诱导补体激活增加。BK股骨柄的钛涂层产生了机械和生物学上有利的界面，这可能有助于增强角膜基质粘附和生物相容性。改善BK PMMA股骨柄的生物相容性可能会改善植入的长期结局。
To evaluate titanium (Ti) sputtering of the poly(methyl methacrylate) (PMMA) stem of the Boston Keratoprosthesis (BK) as a method to enhance interfacial adhesion between the PMMA and the recipient corneal tissue. PMMA specimens were plasma treated with Ar/O2 and coated with Ti using a DC magnetron sputtering instrument. The topography and hydrophilicity of the surfaces were characterized using atomic force microscopy and a water contact angle instrument, respectively. Scratch hardness and adhesion of the Ti film were measured using a mechanical tester. Biocompatibility assessments were performed using cultured human corneal fibroblasts and whole blood ex vivo. The optical quality of the Ti sputtered BK was evaluated using a custom-made optical bench. By contact angle studies, the Ti coating improved PMMA hydrophilicity to match that of medical-grade Ti (Ti-6Al-4V-ELI). Ti sputtering of contact surfaces resulted in a plate-like morphology with increased surface roughness, without impacting the transparency of the BK optical component. Scratch testing indicated that the mechanical behavior of the Ti coating was similar to that of casted Ti, and the coating was stable in pull-off adhesion testing. Sputtered Ti film was highly biocompatible based on tests of cell viability, adhesion, proliferation, differentiation, collagen deposition, and keratocan expression, the properties of which exceeded those of uncoated PMMA and did not induce increased complement activation. Titanium coating of the BK stem generated a mechanically and biologically favorable interface, which may help to enhance corneal stromal adhesion and biocompatibility. Improving the biocompatibility of the BK PMMA stem may improve long-term outcomes of implantation.
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影响因子：
2.8
作者：
Bakshi, Shaunak K.;Paschalis, Eleftherios I.;Chodosh, James
通讯作者：
Chodosh, James
影响因子：
6.4
作者：
Gonzalez-Andrades, Miguel;Sharifi, Roholah;Dohlman, Claes H.
通讯作者：
Dohlman, Claes H.
影响因子：
2.8
作者：
Hou, Joshua H.;de la Cruz, Jose;Djalilian, Ali R.
通讯作者：
Djalilian, Ali R.
影响因子：
3.4
作者：
Jeong, Kyung Jae;Wang, Liqiang;Kohane, Daniel S.
通讯作者：
Kohane, Daniel S.
影响因子：
3.5
作者：
Kaariainen, Tommi O.;Cameron, David C.;Tanttari, Mari
通讯作者：
Tanttari, Mari