Downregulation of inwardly rectifying potassium channel 5.1 expression in C57BL/6J cochlear lateral wall
Downregulation of inwardly rectifying potassium channel 5.1 expression in C57BL/6J cochlear lateral wall
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C57BL/6J 耳蜗侧壁内向整流钾通道 5.1 表达下调
DOI：
10.1007/s11596-016-1600-0
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发表时间：
2016-06
影响因子：
--
通讯作者：
Tao Yan-ling
中科院分区：
文献类型：
--
作者：
Pan Chun-chen;Chu Han-qi;Lai Yan-bing;Sun Yan-bo;Du Zhi-hui;Liu Yun;Chen Jin;Tong Ting;Chen Qing-guo;Zhou Liang-qiang;Bing Dan;Tao Yan-ling
听力损失是老年人最常见的感觉障碍之一。内向整流钾通道5.1（Kir5.1）在调节耳蜗钾循环中起着重要作用，而耳蜗钾循环是听力正常所必需的。研究Kir5.1在C57 BL/6 J小鼠耳蜗中的分布及其与阿勒发病的关系。将40只C57 BL/6 J小鼠随机分为4组，分别为4、12、24和52周龄。免疫荧光法检测Kir5.1的定位。采用实时荧光定量聚合酶链反应（RT-PCR）和蛋白质印迹法检测小鼠耳蜗Kir5.1的mRNA和蛋白表达。Kir5.1在C57 BL/6 J小鼠耳蜗外侧壁螺旋韧带的II型和IV型纤维细胞中均有表达。衰老C57 BL/6 J小鼠耳蜗Kir5.1 mRNA和蛋白表达水平下调。提示C57 BL/6 J小鼠耳蜗外侧壁Kir5.1表达随年龄增加而降低，这可能与其听力损失有关。结果提示Kir5.1在阿勒的发病机制中可能起重要作用。
Age-related hearing loss (AHL) is one of the most common sensory disorders among elderly persons. The inwardly rectifying potassium channel 5.1 (Kir5.1) plays a vital role in regulating cochlear K+circulation which is necessary for normal hearing. The distribution of Kir5.1 in C57BL/6J mice cochleae, and the relationship between the expression of Kir5.1 and the etiology of AHL were investigated. Forty C57BL/6J mice were randomly divided into four groups at 4, 12, 24 and 52 weeks of age respectively. The location of Kir5.1 was detected by immunofluorescence technique. The mRNA and protein expression of Kir5.1 was evaluated in mice cochleae using real-time polymerase-chain reactions (RT-PCR) and Western blotting respectively. Kir5.1 was detected in the type II and IV fibrocytes of the spiral ligament in the cochlear lateral wall of C57BL/6J mice. The expression levels of Kir5.1 mRNA and protein in the cochleae of aging C57BL/6J mice were down-regulated. It was suggested that the age-related decreased expression of Kir5.1 in the lateral wall of C57BL/6J mice was associated with hearing loss. Our results indicated that Kir5.1 may play an important role in the pathogenesis of AHL.
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影响因子：
3.3
作者：
Chen J;Zhao HB
通讯作者：
Zhao HB
影响因子：
5.5
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
2.8
作者：
Ichimiya, I;Suzuki, M;Mogi, G
通讯作者：
Mogi, G
影响因子：
2.5
作者：
Haidi Yang;H. Xiong;Qiuhong Huang;Jiaqi Pang;Xuqing Zheng;Lian Chen;Rongjun Yu;Yiqing Zheng
通讯作者：
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